CAJ | 학술논문

目的:探讨电针疗法减轻缺血脑组织病理损伤的可能作用机理.方法:改良线栓法制备局灶性脑缺血再灌注模型,电针“百会”、“水沟”、“足三里”,通过逆转录聚合酶链反应(RT-PCR)法半定量测定各组Survivin mRNA的表达.结果:电针组病理改变较相同时相模型组病理改变较轻.相同时相电针组Survivin mRNA的表达比模型组均要高,差异均有统计学意义(P<0.05).结论:电针疗法可有效减轻缺血脑组织的病理损伤,其作用机理可能与增加缺血脑组织Survivin mRNA的表达有关.
목적:탐토전침요법감경결혈뇌조직병리손상적가능작용궤리.방법:개량선전법제비국조성뇌결혈재관주모형,전침“백회”、“수구”、“족삼리”,통과역전록취합매련반응(RT-PCR)법반정량측정각조Survivin mRNA적표체.결과:전침조병리개변교상동시상모형조병리개변교경.상동시상전침조Survivin mRNA적표체비모형조균요고,차이균유통계학의의(P<0.05).결론:전침요법가유효감경결혈뇌조직적병리손상,기작용궤리가능여증가결혈뇌조직Survivin mRNA적표체유관.
Objective: To explore possible mechanism of electro-acupuncture therapy on reducing ischemic brain injury. Methods: Modified Suture-occluded method was applied for forming focal cerebral ischemia reperfusion model; the acupuncture points of Electro-acupuncture were "Baihui", "Shuigou" and "Zusanli"; reverse transcriptase polymerase chain reaction (RT-PCR) method was used for semi quantitative determination of serum Survivin mRNA expression. Results: The pathological change in the treatment group was lighter than that model group in the same phase; in the same phase, the Survivin mRNA expression of acupuncture group were higher than that of the model group, with statistically significant differences (P<0.05). Conclusion: Electro-acupuncture therapy can provide effect of reducing ischemic brain injury, the mechanism of which may be associated with the increase of Survivin mRNA expression in ischemic brain tissue.