中医药导报
中醫藥導報
중의약도보
GUIDING JOURNAL OF TCM
2013年
2期
101-102
,共2页
溪黄草%总黄酮%微波辅助提取%正交试验%提取工艺
溪黃草%總黃酮%微波輔助提取%正交試驗%提取工藝
계황초%총황동%미파보조제취%정교시험%제취공예
Rabdosia Serra%Flavonoids%Microwave-assisted extraction%Orthogonal experiment%Extraction process
目的:寻找微波辅助提取甘草黄酮的最佳条件,并对微波辅助提取法(MAE)和超声波提取法(UE)进行对比研究.方法:采用MAE和UE提取甘草黄酮,并用4因素、3水平的正交设计优化微波提取溪黄草总黄酮的实验条件,以分光光度法测定提取液中的总黄酮含量.结果:通过实验发现MAE提取总黄酮的最佳条件为:固液比1∶30,乙醇浓度为65%,加热功率为360 W,加热时间为30 min时,MAE(96.11%)明显优于UE(90.47%).结论:MAE提取率高,且提取时间大大缩短,是一种适合溪黄草总黄酮的提取法.
目的:尋找微波輔助提取甘草黃酮的最佳條件,併對微波輔助提取法(MAE)和超聲波提取法(UE)進行對比研究.方法:採用MAE和UE提取甘草黃酮,併用4因素、3水平的正交設計優化微波提取溪黃草總黃酮的實驗條件,以分光光度法測定提取液中的總黃酮含量.結果:通過實驗髮現MAE提取總黃酮的最佳條件為:固液比1∶30,乙醇濃度為65%,加熱功率為360 W,加熱時間為30 min時,MAE(96.11%)明顯優于UE(90.47%).結論:MAE提取率高,且提取時間大大縮短,是一種適閤溪黃草總黃酮的提取法.
목적:심조미파보조제취감초황동적최가조건,병대미파보조제취법(MAE)화초성파제취법(UE)진행대비연구.방법:채용MAE화UE제취감초황동,병용4인소、3수평적정교설계우화미파제취계황초총황동적실험조건,이분광광도법측정제취액중적총황동함량.결과:통과실험발현MAE제취총황동적최가조건위:고액비1∶30,을순농도위65%,가열공솔위360 W,가열시간위30 min시,MAE(96.11%)명현우우UE(90.47%).결론:MAE제취솔고,차제취시간대대축단,시일충괄합계황초총황동적제취법.
Objective: To explore the best condition of microwave-assisted extraction(MAE) for the flavonoids from Rab-dosia Serra and to compare with traditional ultrasonic extractio (UE). Methods: MAE and UE were adopted to optimize the laboratory conditions of flavonoids extraction by the orthogonal design according to 4 factors and 3 standard,and the flavonoids in Rabdosia Serra was determined with spectrophotometry. Results: Appropriate MAE conditions included liquid/solid ratios of 1:30, ethanol concentration of 65%, the power of microwave oven of 360W, and extraction time of 30min; the recovery of flavonoids from Rabdosia Serra with MAE (96.11%) was higher than UE (90.47%). Conclusion: The experiment results demon-strated higher extracting rate and shorter extracting time. It indicated that MAE was more suitable to extraction of the flavonoids.
