CAJ | 학술논문

本文研究了在不同碳源和氮源以及不同碳氮比的碱性液体培养条件(pH≈10.5)下,嗜碱木素降解菌所产生的木素降解酶的种类和产酶能力.结果表明,Klason木质素有利于MnP的产生,活性达283.50U/L,但在此条件下不产生LiP和Laccase;而蔗糖条件下产生的降解酶Laccase和MnP活性最高,分别为245.42U/L和122.52U/L,而在最佳产酶培养时间它们的活性达到628.03U/L(4天)和129.76U/L(8天).碳源限制有利于酶的产生,而氮源限制则明显地抑制了酶的合成,且后者对菌株的产酶能力影响较大.
본문연구료재불동탄원화담원이급불동탄담비적감성액체배양조건(pH≈10.5)하,기감목소강해균소산생적목소강해매적충류화산매능력.결과표명,Klason목질소유리우MnP적산생,활성체283.50U/L,단재차조건하불산생LiP화Laccase;이자당조건하산생적강해매Laccase화MnP활성최고,분별위245.42U/L화122.52U/L,이재최가산매배양시간타문적활성체도628.03U/L(4천)화129.76U/L(8천).탄원한제유리우매적산생,이담원한제칙명현지억제료매적합성,차후자대균주적산매능력영향교대.
The article research ligninolytic enzymes production and activities of alkaliphilic ligninolytic bacteria in alkaline liquid medium(pH≈10.5) with various carbons or nitrogen and various ratio of carbons and nitrogen. Klason-lignin takes advantage of production of MnP, the activity is 283.50U/L, but LiP and Laccase don’t detected. The activities of Laccase and MnP are higher in sugar cultivation condition, their activities are 245.42U/L and 122.52U/L respectively. And their activities are 628.03U/L(4days) and 129.76U/L(8days) respectively at optimal cultivation times. Lower carbon medium is suitable for production, and low nitrogen medium restrain obviously synthesis of the enzymes.