化学通报（网络版）
化學通報（網絡版）
화학통보（망락판）
Chemistry Online
2003年
1期
,共1页
偶联剂%标记蛋白质%合成%放射性免疫分析
偶聯劑%標記蛋白質%閤成%放射性免疫分析
우련제%표기단백질%합성%방사성면역분석
Conjugate reagent%Labell-protein%Synthesis%Redioimmunoassay
用亲电取代法通过异烟酸与氧化汞直接反应,合成汞代吡啶羧酸再进行酯化,制得一种适合于各种蛋白质碘标记的偶联剂:氯化汞代异烟酸-N-丁二酰亚胺酯.该试剂结合固相分离技术来建立一简便的标记抗体途径.其蛋白质标记率可达40%~60%.
用親電取代法通過異煙痠與氧化汞直接反應,閤成汞代吡啶羧痠再進行酯化,製得一種適閤于各種蛋白質碘標記的偶聯劑:氯化汞代異煙痠-N-丁二酰亞胺酯.該試劑結閤固相分離技術來建立一簡便的標記抗體途徑.其蛋白質標記率可達40%~60%.
용친전취대법통과이연산여양화홍직접반응,합성홍대필정최산재진행지화,제득일충괄합우각충단백질전표기적우련제:록화홍대이연산-N-정이선아알지.해시제결합고상분리기술래건립일간편적표기항체도경.기단백질표기솔가체40%~60%.
By virtue of isonicotinic acid as precursor compound, N-saccinimidy lisonicotinizute has been synthesized through eletrophilic reaction and then being esterified, one kind of conjugate reagent adapted to all kinds of protein can be obtained. Moreover, a quick and convenient way of labell antibody has been estabished, combining this conjugate reagent with the technique of the fixed phrase separation. The rate of its protein labell thus can reach 40%~60% averagely.
