CAJ | 학술논문

酶是生物合成与转化的关键因素,酶催化性能既取决于酶蛋白分子自身结构,又取决于介质微环境.非水酶学和胶束酶学是近二三十年来兴起的酶学研究新领域,主要研究有机溶剂和反胶束中酶的催化性能并探索其潜在应用.表面活性剂既是区分这两个分支领域的标志,又是联系两者的桥梁.本文在对这两个领域的研究概况作一简要介绍的同时,着重介绍了表面活性剂修饰酶、微乳液凝胶固定化酶等研究进展.
매시생물합성여전화적관건인소,매최화성능기취결우매단백분자자신결구,우취결우개질미배경.비수매학화효속매학시근이삼십년래흥기적매학연구신영역,주요연구유궤용제화반효속중매적최화성능병탐색기잠재응용.표면활성제기시구분저량개분지영역적표지,우시련계량자적교량.본문재대저량개영역적연구개황작일간요개소적동시,착중개소료표면활성제수식매、미유액응효고정화매등연구진전.
Enzyme plays a key role in biosynthesis and biotransformation. Its catalytic properties depend on the structure of enzymic protein as well as on the microenvironment it experienced. Nonaqueous Enzymology and Micellar Enzymology are new branches of enzymology in recent decades, the research aim is to study the effect of organic solvent and reverse micelles on the catalytic properties of an enzyme. Surfactant, amphile, is viewed as both the mark to distinguish between them and the bridge to connect them. In addition to make a brief introduction of these two research fields, this review article puts emphasis on recent advances in modification of enzyme by surfactant and immobilization of enzyme in water-in-oil microemulsion.