化学通报（网络版）
化學通報（網絡版）
화학통보（망락판）
Chemistry Online
2005年
1期
1-4
,共4页
固相萃取%高效液相色谱-质谱%螺旋藻%微囊藻毒素
固相萃取%高效液相色譜-質譜%螺鏇藻%微囊藻毒素
고상췌취%고효액상색보-질보%라선조%미낭조독소
Solid-phase extraction%HPLC-electrospray ionization mass spectrometry%Spirulina%Microcystins
通过超声提取、固相萃取纯化、反相高效液相色谱-电喷雾电离质谱联用技术测定螺旋藻保健品中的微囊藻毒素-RR、-YR、-LR.采用选择离子监测质荷比(m/Z)为520.3、1046.1、996.9的带正电荷的分子离子峰进行定量分析.该法检测限为0.05μg/g,线性定量范围0.1~8μg/g藻粉.此方法准确、灵敏度高、专属性好,可作为食品中蓝藻毒素的分析方法,为食品安全风险评价提供依据.
通過超聲提取、固相萃取純化、反相高效液相色譜-電噴霧電離質譜聯用技術測定螺鏇藻保健品中的微囊藻毒素-RR、-YR、-LR.採用選擇離子鑑測質荷比(m/Z)為520.3、1046.1、996.9的帶正電荷的分子離子峰進行定量分析.該法檢測限為0.05μg/g,線性定量範圍0.1~8μg/g藻粉.此方法準確、靈敏度高、專屬性好,可作為食品中藍藻毒素的分析方法,為食品安全風險評價提供依據.
통과초성제취、고상췌취순화、반상고효액상색보-전분무전리질보련용기술측정라선조보건품중적미낭조독소-RR、-YR、-LR.채용선택리자감측질하비(m/Z)위520.3、1046.1、996.9적대정전하적분자리자봉진행정량분석.해법검측한위0.05μg/g,선성정량범위0.1~8μg/g조분.차방법준학、령민도고、전속성호,가작위식품중람조독소적분석방법,위식품안전풍험평개제공의거.
A high performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) method has been developed for the determination of microcystin (MCYST) variants in spirulina samples. The samples were extracted with 5%acetic acid in water, cleanup with a reversed-phase solid-phase extraction cartridge, and then subjected to the HPLC-MS in the selected ion monitoring (m/Z) 520.3,1046.1,996.9 for specific measurement of MCYST variant-RR,-YR,-LR in the concentration range of 0.1 to 8μg·g-1. The lower limit of detection was found to be around 0.05μg·g-1. The method is highly selective and sensitive for the analysis of trace amounts MCYSTs and is adequate for analysis of MCYSTs in spirulina health food products.
