CAJ | 학술논문

用荧光光谱法研究了阳离子水溶性聚芴(Poly[(9,9-bis(3’-(N,N-dimethyl)-N-ethylammonium)propyl)--2,7-fluorene)-alt-1,4-phenylene]Dibromide(PF))与生物大分子牛血清白蛋白(BSA)之间的相互作用,并进一步研究了酸度和离子强度对二者相互作用的影响.结果表明,极微量的BSA便会使PF的荧光产生可分辨的降低,改变蛋白质BSA的表面电荷,BSA对PF的猝灭效率也发生较大变化;而PF可猝灭BSA的荧光,同时自身的荧光增强并存在一个等发射点,说明BSA和PF之间存在相互作用,二者之间发生了能量转移.
용형광광보법연구료양리자수용성취물(Poly[(9,9-bis(3’-(N,N-dimethyl)-N-ethylammonium)propyl)--2,7-fluorene)-alt-1,4-phenylene]Dibromide(PF))여생물대분자우혈청백단백(BSA)지간적상호작용,병진일보연구료산도화리자강도대이자상호작용적영향.결과표명,겁미량적BSA편회사PF적형광산생가분변적강저,개변단백질BSA적표면전하,BSA대PF적졸멸효솔야발생교대변화;이PF가졸멸BSA적형광,동시자신적형광증강병존재일개등발사점,설명BSA화PF지간존재상호작용,이자지간발생료능량전이.
The interaction between the conjugated polymer Poly[(9,9-bis(3’-(N,N-dimethyl)-N-ethylammonium)-propyl)-2,7-fluorene)-alt-1,4-phenylene]Dibromide(PF) and biomocule bovine serum albumin (BSA), as well as the influence of the acidity and the ion intensity on the interaction between them, were studied by fluorescence spectroscopic technique. The results showed that the addition of infinitesimal BSA can decrease the fluorescence intensity of PF. When the surface charge of BSA was changed, the quenching efficiency was also altered. Meanwhile, the fluorescence of BSA can be quenched with the addition of PF and the fluorescence intensity PF itself increased. The spectra displayed iso emission point at 394.5nm and demonstrated that there existed interaction and energy transfer between PF and BSA.