化学通报（网络版）
化學通報（網絡版）
화학통보（망락판）
Chemistry Online
2008年
1期
,共1页
微生物代谢组学%样品前处理%生物量培养%淬灭%代谢物提取
微生物代謝組學%樣品前處理%生物量培養%淬滅%代謝物提取
미생물대사조학%양품전처리%생물량배양%쉬멸%대사물제취
Microbial metabolome%Sample preparation%Biomass cultivation%Quenching%Extraction
微生物代谢组学是指全面分析(定性和定量)细胞生长或生产周期某一时刻细胞内和细胞周围的所有低分子量代谢物.微生物代谢组学研究需要可靠和重现地分析细胞内较宽动力学浓度范围(nmol~mmol)、化学功能各异的代谢产物,因此,需要对从生物量培养、灭活和代谢产物的提取到代谢物的定量分析这整个实验过程提出耐用、可重现和可靠的实验方案.本文介绍了灭活过程中细胞内代谢产物泄漏的处理方法,较为详细地论述了通过灭活草案捕获代谢反应活性方法和代谢物提取方法的优化,并对微生物代谢组学样品前处理的目前发展趋势提出初步见解.
微生物代謝組學是指全麵分析(定性和定量)細胞生長或生產週期某一時刻細胞內和細胞週圍的所有低分子量代謝物.微生物代謝組學研究需要可靠和重現地分析細胞內較寬動力學濃度範圍(nmol~mmol)、化學功能各異的代謝產物,因此,需要對從生物量培養、滅活和代謝產物的提取到代謝物的定量分析這整箇實驗過程提齣耐用、可重現和可靠的實驗方案.本文介紹瞭滅活過程中細胞內代謝產物洩漏的處理方法,較為詳細地論述瞭通過滅活草案捕穫代謝反應活性方法和代謝物提取方法的優化,併對微生物代謝組學樣品前處理的目前髮展趨勢提齣初步見解.
미생물대사조학시지전면분석(정성화정량)세포생장혹생산주기모일시각세포내화세포주위적소유저분자량대사물.미생물대사조학연구수요가고화중현지분석세포내교관동역학농도범위(nmol~mmol)、화학공능각이적대사산물,인차,수요대종생물량배양、멸활화대사산물적제취도대사물적정량분석저정개실험과정제출내용、가중현화가고적실험방안.본문개소료멸활과정중세포내대사산물설루적처리방법,교위상세지논술료통과멸활초안포획대사반응활성방법화대사물제취방법적우화,병대미생물대사조학양품전처리적목전발전추세제출초보견해.
Microbial metabolome refers to the comprehensive (qualitative and quantitative) analysis of the complete set of all low molecular weight metabolites present in and around growing cells at a given time during their growth or production cycle. Investigations in microbial metabolome require reliable and reproducible analysis of the metabolites in the cell across a broad dynamic range of concentrations (nmol to mmol) and from a range of different chemical functionalities. The development of robust and reliable experimental protocols for all steps in the experimental procedure, ranging from biomass cultivation, quenching, and extraction of the metabolome to the quantitative analysis of the metabolites, is required. In this paper, the methods for handling the leakage of cellular metabolites during quenching were introduced, and optimizing the methods for arresting metabolic activity by quenching protocols and the extraction methods for intracellular metabolites were discussed;current trends in sample preparation for microbial metabolome analysis were also briefly addressed.
