CAJ | 학술논문

采用高效液相色谱法以对羟基苯甲酸丁酯作为内标定量测定了鸡肉中己烯雌酚的含量.色谱柱为C18柱,0.02 mol/L三乙胺水溶液(加磷酸调pH=3.0)-乙腈(v/v=55/45)作为流动相,采用等度洗脱,检测波长为240 nm.结果显示,己烯雌酚的线性范围为0.324~1.30μg/mL,相关系数为0.9998,用浓度为0.648μg/mL的标准溶液重复进样6次,其相对标准偏差(RSD)为1.28%.方法简便准确,适于鸡肉组织中违禁药物己烯雌酚含量的测定.
채용고효액상색보법이대간기분갑산정지작위내표정량측정료계육중기희자분적함량.색보주위C18주,0.02 mol/L삼을알수용액(가린산조pH=3.0)-을정(v/v=55/45)작위류동상,채용등도세탈,검측파장위240 nm.결과현시,기희자분적선성범위위0.324~1.30μg/mL,상관계수위0.9998,용농도위0.648μg/mL적표준용액중복진양6차,기상대표준편차(RSD)위1.28%.방법간편준학,괄우계육조직중위금약물기희자분함량적측정.
The HPLC method was established for the determination of diethylstilbestrol in chicken meat. Diethylstilbestrol and internal standard butyl paraben were separated on a C18 column and detected at 240nm. The mobile phase consisted of 40%actonitrile and 0.02 mol/L trimethylamine (adjust pH to 3.0 by phosphoric acid). Result of the standard curve method is accorded with that of internal standard method. The concentration of diethylstilbestrol was calculated by the internal standard method. The linearity between peak area and concentration of diethylstilbestrol existed in the range of 0.324~1.30 μg/mL and RSD of peak area was in the level of 1.28%. The recovery of diethylstilbestrol was from 98.8%~101.2%(n=3). The method is stable, practical and suitable for the quantitative determination of diethylstilbestrol in chicken meat.