CAJ | 학술논문

采用实验生态学的方法,研究了大型海藻鼠尾藻(Sargassum thunbergii)对赤潮微藻中肋骨条藻(Skeletonema costatum)的克生效应及其对 CO2加富的响应变化.结果表明,正常培养的鼠尾藻新鲜组织、干粉末和水溶性抽提液能够导致中肋骨条藻的细胞密度显著降低(P<0.05), CO2加富培养的鼠尾藻新鲜组织、干粉末和水溶性抽提液能够更加明显地抑制中肋骨条藻种群生长(P<0.01).实验结果证实鼠尾藻对中肋骨条藻具有克生效应,而 CO2加富培养引起了鼠尾藻对中肋骨条藻克生效应的变化,使其对中肋骨条藻生长的抑制作用显著加强.正常培养的鼠尾藻培养水过滤液能够显著抑制中肋骨条藻的生长(P<0.05),而 CO2加富培养的鼠尾藻培养水过滤液对中肋骨条藻的生长没有表现出明显的影响(P>0.05),因此推测 CO2加富培养可能改变了鼠尾藻对中肋骨条藻克生作用的方式,作用方式由分泌克生物质转变为细胞直接接触转递.
채용실험생태학적방법,연구료대형해조서미조(Sargassum thunbergii)대적조미조중륵골조조(Skeletonema costatum)적극생효응급기대 CO2가부적향응변화.결과표명,정상배양적서미조신선조직、간분말화수용성추제액능구도치중륵골조조적세포밀도현저강저(P<0.05), CO2가부배양적서미조신선조직、간분말화수용성추제액능구경가명현지억제중륵골조조충군생장(P<0.01).실험결과증실서미조대중륵골조조구유극생효응,이 CO2가부배양인기료서미조대중륵골조조극생효응적변화,사기대중륵골조조생장적억제작용현저가강.정상배양적서미조배양수과려액능구현저억제중륵골조조적생장(P<0.05),이 CO2가부배양적서미조배양수과려액대중륵골조조적생장몰유표현출명현적영향(P>0.05),인차추측 CO2가부배양가능개변료서미조대중륵골조조극생작용적방식,작용방식유분비극생물질전변위세포직접접촉전체.
The allelopathic effect of Sargassum thunbergii on Skeletonema costatum and its response to CO2 enrichment were investigated by an experimental ecology method. Results showed that, under normal conditions, fresh tissue, dry power and aqueous extracts of S. thunbergii resulted in remarkably reductions in cell density of S. costatum (P<0.05). In addition, the fresh tissue, dry power and aqueous extracts of S. thunbergii, which were cultured under CO2 enrichment conditions, led to more significant decreases in cell density of S. costatum (P<0.01). Under normal conditions, the culture media filtrate of S. thunbergii drastically inhibited the growth of S. costatum (P<0.05), while that of S. thunbergii which were cultured under CO2 enrichment conditions did not exhibit significantly inhibitive effect on S. costatum (P>0.05). Therefore, it was speculated that the mediating mechanism of the allelopathic effect might be changed by CO2 enrich-ment, from the secreted allelopathic substances to direct cell contact.