CAJ | 학술논문

利用常规 PCR 未纯化产物直接测序的方法,获得紫红笛鲷(Lutjanus argentimaculatus)全长为16543 bp 线粒体 DNA 全序列, GenBank 登录号为 JN182927.结合 GenBank 中的已有数据比较分析表明:紫红笛鲷与笛鲷属(Lutjanus)线粒体基因组成基本一致,符合脊椎动物的特征,各基因间进化速率差异显著;13个蛋白编码基因拼接序列贝叶斯建树的后验率显著高于单基因或全序列,更适用于笛鲷属的贝叶斯法系统分化分析和年代推断;13个蛋白编码基因及其拼接序列进行的系统进化分析均支持紫红笛鲷与勒氏笛鲷(Lutjanus russellii)及海鸡母笛鲷(Lutjanus rivulatus)亲缘关系较近;基于13个蛋白编码基因拼接序列的贝叶斯松散分子钟推算出笛鲷属鱼类两个主要支系大约在61.5Ma 前古新世的达宁阶与蒙蒂阶交界时期发生分化.
이용상규 PCR 미순화산물직접측서적방법,획득자홍적조(Lutjanus argentimaculatus)전장위16543 bp 선립체 DNA 전서렬, GenBank 등록호위 JN182927.결합 GenBank 중적이유수거비교분석표명:자홍적조여적조속(Lutjanus)선립체기인조성기본일치,부합척추동물적특정,각기인간진화속솔차이현저;13개단백편마기인병접서렬패협사건수적후험솔현저고우단기인혹전서렬,경괄용우적조속적패협사법계통분화분석화년대추단;13개단백편마기인급기병접서렬진행적계통진화분석균지지자홍적조여륵씨적조(Lutjanus russellii)급해계모적조(Lutjanus rivulatus)친연관계교근;기우13개단백편마기인병접서렬적패협사송산분자종추산출적조속어류량개주요지계대약재61.5Ma 전고신세적체저계여몽체계교계시기발생분화.
We got 16 543 bp Red snapper (Lutjanus argentimaculatus) complete mitochondrial DNA sequence through common PCR, with the GenBank registration number being JN182927. Our sequence and the data in Gen-Bank, red snapper’s mitochondrion gene composition were consistent with other snappers, according to the charac-teristics of vertebrates. Bayesian tree of complete sequence and 13 protein-coding genes and their splicing sequence indicated that: red snapper was close to L. russellii and L. rivulatus. The evolution rate differences of different mi-tochondrial sections were obvious. COX 1, ND 2 and ATPase6 Bayesian posterior rates were significantly higher than the other ten in 13 protein-coding mitochondrial genes of. The 13 protein-coding gene splicing sequences Bayesian posterior rates were significantly higher than completed sequence, and were more suitable for Bayesian uncorrected relaxed lognormal clock analysis. Thirteen protein coding gene splicing sequence’s Bayesian uncor-rected relaxed lognormal clock work out snappers’ two main branches broke out in 61.5 Ma before, which was the junction point of Danian and Selandian in Paleocene.