CAJ | 학술논문

作者采用2 mL 的冷存管和程序降温仪高效超低温保存美洲黑石斑(Centropristis striata L.)精子.分析比较了4种不同浓度抗冻剂15%DMSO(二甲基亚砜)、15%EG(乙二醇)、15%PG(丙二醇)和10%Meth(甲醇),5种降温速率(10,15,20,25,30℃/min)和5种解冻温度(30,35,40,45,50℃)对美洲黑石斑精子超低温保存效果的影响.实验结果表明,采用 Hanks’作为稀释液,15% DMSO、15% EG、15% PG 作为抗冻剂,30℃/min 的降温速率对精液进行超低温保存35℃水浴解冻,冻精激活后获得理想的运动率(>60%).通过对抗冻剂、降温速率、解冻温度的筛选,作者建立了美洲黑石斑精液高效超低温保存的方法,并对冻精进行长期保存.美洲黑石斑精子超低温保存方法的建立对于海水鱼类精子库的建立以及种质资源的保存和生物多样性的保护具有重要意义.
작자채용2 mL 적랭존관화정서강온의고효초저온보존미주흑석반(Centropristis striata L.)정자.분석비교료4충불동농도항동제15%DMSO(이갑기아풍)、15%EG(을이순)、15%PG(병이순)화10%Meth(갑순),5충강온속솔(10,15,20,25,30℃/min)화5충해동온도(30,35,40,45,50℃)대미주흑석반정자초저온보존효과적영향.실험결과표명,채용 Hanks’작위희석액,15% DMSO、15% EG、15% PG 작위항동제,30℃/min 적강온속솔대정액진행초저온보존35℃수욕해동,동정격활후획득이상적운동솔(>60%).통과대항동제、강온속솔、해동온도적사선,작자건립료미주흑석반정액고효초저온보존적방법,병대동정진행장기보존.미주흑석반정자초저온보존방법적건립대우해수어류정자고적건립이급충질자원적보존화생물다양성적보호구유중요의의.
In the present study, Centropristis striata sperm was efficiently cryopreserved using a programmable freezer. The motility of both fresh and post-thaw sperm was investigated in order to optimize the sperm cryopre-servation protocols for C. striata. Four cryoprotectants (15% dimethyl sulfoxide, 15% ethylene glycol, 15% pro-pylene glycol and 10% methanol), five cooling rates (10, 15, 20, 25 and 30℃/min) as well as five thawing tem-peratures (30, 35, 40, 45 and 50 ℃) were designed and tested in the sperm cryopreservation, and their effects on post-thaw sperm motility were studied. Optimal post-thaw motility (>60%) were achieved when using Hanks’s supplemented with 15% DMSO, 15% PG and 15% EG with 30℃/min cooling rate, and 35℃ water bath. An effi-cient cryopreservation method for C. striata sperm was established by analyzing the effect of cryoprotectants, cooling rates and thaw temperatures. This method is advantageous not only to establish laboratory experiment but also to preserve genetic resources for routine aquaculture hatchery operation.