CAJ | 학술논문

目的:通过对口腔扁平苔藓(OLP)局部组织及外周血中 IL-2、IL-10表达进行研究,进一步探讨 OLP 发病机制.方法:应用免疫组织化学 SABC 法检测30例 OLP 中 IL-2、IL-10的表达数量及分布,同时用 ELISA 法检测外周血中 IL-2、IL-10的含量.结果:①在病损组织中,OLP 组中 IL-2、IL-10表达显著高于对照组(P <0.05). IL-2主要分布在淋巴细胞浸润带,在基底膜附近较集中;IL-10主要分布在固有层,远离基底膜.②在外周血中,糜烂型 OLP 组 IL-2、IL-10含量明显高于对照组和非糜烂型 OLP 组(P <0.05).结论:①根据 IL-2、IL-10在 OLP 中表达和分布情况,提示IL-2、IL-10在 OLP 发病机制中参与了机体免疫的调节和介导作用.②外周血中 IL-10高表达,提示糜烂型 OLP 患者全身的免疫反应参与了本病的形成或可能伴有全身免疫系统功能紊乱.
목적:통과대구강편평태선(OLP)국부조직급외주혈중 IL-2、IL-10표체진행연구,진일보탐토 OLP 발병궤제.방법:응용면역조직화학 SABC 법검측30례 OLP 중 IL-2、IL-10적표체수량급분포,동시용 ELISA 법검측외주혈중 IL-2、IL-10적함량.결과:①재병손조직중,OLP 조중 IL-2、IL-10표체현저고우대조조(P <0.05). IL-2주요분포재림파세포침윤대,재기저막부근교집중;IL-10주요분포재고유층,원리기저막.②재외주혈중,미란형 OLP 조 IL-2、IL-10함량명현고우대조조화비미란형 OLP 조(P <0.05).결론:①근거 IL-2、IL-10재 OLP 중표체화분포정황,제시IL-2、IL-10재 OLP 발병궤제중삼여료궤체면역적조절화개도작용.②외주혈중 IL-10고표체,제시미란형 OLP 환자전신적면역반응삼여료본병적형성혹가능반유전신면역계통공능문란.
Objective:To evaluate the expression of IL-2,IL-10 in oral lichen planus (OLP) and to evaluate the possible pathogenesis of oral lichen planus. Method:Immunohistochemical technique was employed to study the expression of IL-2,IL-10 in oral mucosa and ELASA was used for detecting the contents of IL-2,IL-10 in peripheral blood of 30 OLP cases. Result:The expression of IL-2,IL-10 were significantly increased in OLP compared with that in NOM(P <0.05). IL-2 was mainly localized in the lymphocytic infiltration,focus on the basal cell membrane and IL-10 was mainly localized in lamina propria separately. The amount of IL-10 was obviously elevated in erosion OLP peripheral blood compared with the non-erosion and NOM(P <0.05). Conclusion:The increasing expression of IL-2,IL-10 has also explained the association of these cytokines with Local anomaly Immune accommodation and Immune-Induced effects. The levels of IL-10 illustrated patients with OLP erosion might accompany with disorder of immune system or the immunity was associated with the cause of this disease.