CAJ | 학술논문

目的:探讨三苯氧胺(Tamoxifen,TAM)对人舌鳞癌细胞系 Tca8113的增殖抑制作用.方法:不同浓度 TAM处理体外培养的人舌鳞癌细胞 Tca8113,MTT 法检测细胞增殖,倒置显微镜下观察细胞形态学变化.结果:在浓度为1、2、5、10μmol/L 时,TAM 呈现出剂量依赖性抑制人舌鳞癌细胞 Tca8113的增殖作用.通过对三个不同浓度(2μmol/L、5μmol/L、10μmol/L)TAM 实验组的抑制率进行比较,差异有统计学意义(P <0.05),细胞抑制率与药物浓度呈正相关.细胞超微结构显示,药物浓度与细胞结构呈剂量依赖关系.结论:TAM 可显著抑制人舌鳞癌细胞 Tca8113的增殖,其作用呈剂量依赖关系.
목적:탐토삼분양알(Tamoxifen,TAM)대인설린암세포계 Tca8113적증식억제작용.방법:불동농도 TAM처리체외배양적인설린암세포 Tca8113,MTT 법검측세포증식,도치현미경하관찰세포형태학변화.결과:재농도위1、2、5、10μmol/L 시,TAM 정현출제량의뢰성억제인설린암세포 Tca8113적증식작용.통과대삼개불동농도(2μmol/L、5μmol/L、10μmol/L)TAM 실험조적억제솔진행비교,차이유통계학의의(P <0.05),세포억제솔여약물농도정정상관.세포초미결구현시,약물농도여세포결구정제량의뢰관계.결론:TAM 가현저억제인설린암세포 Tca8113적증식,기작용정제량의뢰관계.
Objective:To investigate the inhibiting effect of tamoxifen on proliferation of human tongue carcinoma cell line Tca8113. Method:Human tongue carcinoma cell line Tca8113 was incubated with different concentrations of tamoxifen, cell proliferation was evaluated by MTT method. The morphological changes of cells in each group were observed with in-verted microscope. Result:After the cells being treated with tamoxifen at different concentration(1,2,5,10 μmol/L)for 24 hours,the inhibition rate(%) were(3.42±0.38),(15.61±2.47),(44.54±0.58) and(61.48±0.69) respectively. Tamoxifen in-hibited proliferation significantly (P <0.05)in dose-depended manner. The changes of cellular ultrastructure were relative with the concentration of tamoxifen. Conclusion:Tamoxifen can significantly inhibit human tongue carcinoma cell line Tca8113's proliferation in dose-depended manner.