CAJ | 학술논문

目的:了解草乌甲素对佐剂性关节炎(AA)大鼠原发性关节炎的抗炎镇痛作用.方法:用Freund's完全佐剂免疫Wistar大鼠建立AA动物模型,将其随机分为3组,即草乌甲素治疗2组(剂量分别为100μg·kg-1、50μg·kg-1)、注射用水组.于AA动物模型建立当天对实验大鼠进行处理,连续7d,并记录各组大鼠体重、右踝关节周径变化.治疗结束当天记录炎侧踝关节屈关节疼痛试验评分,用ELISA法检测大鼠血清的TNF-α、INF-γ、PGE2、β-内啡肽含量.结果:在100μg·kg-1治疗组中,草乌甲素在治疗第2天即可明显减轻关节肿胀(P<0.05),提高AA大鼠痛阈(p<0.05),显著降低AA大鼠PGE2含量、提升血清β-内啡肽含量(P<0.01);在50μg·kg-1治疗组中,草乌甲素从治疗的第3天起开始减轻关节肿胀(P<0.05),对大鼠PGE2含量、提升血清β-内啡肽含量作用较弱(P >0.05).100μg·kg-1、50μg·kg-1草乌甲素均可降低AA大鼠血清TNF-α含量(P<0.01),但两者间无统计学差异;草乌甲素对血清INF-γ无明显影响(P >0.05).结论:草乌甲素明显改善AA大鼠关节的肿胀,这可能与其降低血清TNF-α、PGE2含量有关;草乌甲素提高AA大鼠痛阈可能与降低血清PGE2,提升血清β-内啡肽有关.
목적:료해초오갑소대좌제성관절염(AA)대서원발성관절염적항염진통작용.방법:용Freund's완전좌제면역Wistar대서건립AA동물모형,장기수궤분위3조,즉초오갑소치료2조(제량분별위100μg·kg-1、50μg·kg-1)、주사용수조.우AA동물모형건립당천대실험대서진행처리,련속7d,병기록각조대서체중、우과관절주경변화.치료결속당천기록염측과관절굴관절동통시험평분,용ELISA법검측대서혈청적TNF-α、INF-γ、PGE2、β-내배태함량.결과:재100μg·kg-1치료조중,초오갑소재치료제2천즉가명현감경관절종창(P<0.05),제고AA대서통역(p<0.05),현저강저AA대서PGE2함량、제승혈청β-내배태함량(P<0.01);재50μg·kg-1치료조중,초오갑소종치료적제3천기개시감경관절종창(P<0.05),대대서PGE2함량、제승혈청β-내배태함량작용교약(P >0.05).100μg·kg-1、50μg·kg-1초오갑소균가강저AA대서혈청TNF-α함량(P<0.01),단량자간무통계학차이;초오갑소대혈청INF-γ무명현영향(P >0.05).결론:초오갑소명현개선AA대서관절적종창,저가능여기강저혈청TNF-α、PGE2함량유관;초오갑소제고AA대서통역가능여강저혈청PGE2,제승혈청β-내배태유관.
Objective:To evaluate the effect of bul eyaconitine A(BLA) on adjuvant arthritis rats. Method:AA Wistar rats were induced by Freund’s complete adjuvant,Beginning from the onset of AA,AA Wistar rats were randomly assigned to bul eyaconitine A (at a dose of 50μg/kg (n=12),or 100μg/kg (n=12) ),or Aqua pro injection(at a dose of 100μL, n=12), once daily, respectively, for seven days.Increased percentage of body weight, change of circumference of ancle, and scores of arthritic flexion pain test were evaluated. The levels of TNF-α,INF-γ,PGE2 andβ-EP were measured with the use of ELISA. Result:A 100μg/kg of BLA can significantly reduce the circumference of arthritic ankle starting from the 2nd day (p<0.05), and the same result occurred at 50μg/kg of BLA from the 3rd day (p<0.05). BLA produced significant analgesia at a dose of 100μg/kg (p<0.05). The levels of TNF-α and PGE2 increased in 100μg/kg BLA treated rats (p<0.01). The levels of TNF-α in rats treated with 50μg/kg BLA decreased significantly as compared with Aqua pro injection group (p<0.01), and there was no difference between 50μg/kg group and 100μg/kg group.Conclusion:BLA can improve the inflammation of AA rats, which was correlated with its ability of reducing the TNF-αand PGE2 levels in serum. BLA can also reduce inflammatory pain by reducing the level of PGE2 and increasing the level ofβ-EP in serum.