CAJ | 학술논문

采用液液萃取净化的前处理方法,建立了用甲醇-乙腈-水(40∶5∶55体积比)三元流动相体系分离黄曲霉毒素(G2、G1、B2、B1),柱后碘衍生、高效液相色谱荧光检测器测定粮食中黄曲霉毒素的新方法.使用该方法可在24 min内完成测定,4种黄曲霉毒素的线性关系r值均大于0.9995.样品在不同水平的加标回收试验中,回收率为80.3%~97.0%,相对标准偏差2.1%~4.0%,黄曲霉毒素G2、G1、B2、B1的检出限均小于0.40μg/kg.该方法具有样品前处理简单、操作稳定、试剂消耗少、成本低、准确且重复性好等优点,适用于粮食中黄曲霉毒素的定性定量分析.
채용액액췌취정화적전처리방법,건립료용갑순-을정-수(40∶5∶55체적비)삼원류동상체계분리황곡매독소(G2、G1、B2、B1),주후전연생、고효액상색보형광검측기측정양식중황곡매독소적신방법.사용해방법가재24 min내완성측정,4충황곡매독소적선성관계r치균대우0.9995.양품재불동수평적가표회수시험중,회수솔위80.3%~97.0%,상대표준편차2.1%~4.0%,황곡매독소G2、G1、B2、B1적검출한균소우0.40μg/kg.해방법구유양품전처리간단、조작은정、시제소모소、성본저、준학차중복성호등우점,괄용우양식중황곡매독소적정성정량분석.
A new method for aflatoxin G2,G1,B2 and B1 in grain by post-column derivatization with iodine and high performance liquid chromatography-fluorescence detection was developed.Liquid-liquid extraction was used for sample cleanup and full sep-aration of 4 aflatoxins could be achieved by the mobile phase composed of methanol-acetonitrile-water(40∶5∶55,V/V) within 24 minutes. The average correlation of calibration curve was of 0.9995.Recoveries of samples at several spiked levels were ranged between 80.3%and 97.0%,and the relative standard deviation(RSD) was 2.1%~4.0%. The limit of determination(LOD) of afla-toxins G2,G1,B2 and B1 were below 0.40μg/kg.This method has advantages of simple process of sample treatment,low consump-tion of reagents,low cost,rapid and exact,and is suitable for the quantitative and qualitative determination of aflatoxin in grain.