粮食与油脂
糧食與油脂
양식여유지
CEREALS & OILS
2013年
1期
22-25
,共4页
花生壳%水溶性膳食纤维%大孔树脂纯化
花生殼%水溶性膳食纖維%大孔樹脂純化
화생각%수용성선식섬유%대공수지순화
peanut null%soluble dietary fiber%macroporous resin purification
选取8种大孔吸附树脂进行静态吸附性能研究,筛选出最有效D101树脂分离纯化花生壳水溶性膳食纤维.通过动态吸附性能研究,确定应用D101树脂分离纯化花生壳水溶性膳食纤维优化工艺条件;在上样流速2 ml/min、上样液质量浓度为1 mg/mL~2 mg/mL、上样液pH为10左右条件下吸附较强;在洗脱剂乙醇体积分数为70%、洗脱液流速为1 ml/min洗脱时洗脱效果最好.经紫外光谱扫描及SDF纯化物纯度测定结果显示,经纯化后花生壳水溶性膳食纤维纯度较高,可达96.4%;且蛋白含量较少,仅为0.23%.
選取8種大孔吸附樹脂進行靜態吸附性能研究,篩選齣最有效D101樹脂分離純化花生殼水溶性膳食纖維.通過動態吸附性能研究,確定應用D101樹脂分離純化花生殼水溶性膳食纖維優化工藝條件;在上樣流速2 ml/min、上樣液質量濃度為1 mg/mL~2 mg/mL、上樣液pH為10左右條件下吸附較彊;在洗脫劑乙醇體積分數為70%、洗脫液流速為1 ml/min洗脫時洗脫效果最好.經紫外光譜掃描及SDF純化物純度測定結果顯示,經純化後花生殼水溶性膳食纖維純度較高,可達96.4%;且蛋白含量較少,僅為0.23%.
선취8충대공흡부수지진행정태흡부성능연구,사선출최유효D101수지분리순화화생각수용성선식섬유.통과동태흡부성능연구,학정응용D101수지분리순화화생각수용성선식섬유우화공예조건;재상양류속2 ml/min、상양액질량농도위1 mg/mL~2 mg/mL、상양액pH위10좌우조건하흡부교강;재세탈제을순체적분수위70%、세탈액류속위1 ml/min세탈시세탈효과최호.경자외광보소묘급SDF순화물순도측정결과현시,경순화후화생각수용성선식섬유순도교고,가체96.4%;차단백함량교소,부위0.23%.
Eight kinds of macroporous resins adsorption property had been investigated in isolating and purifying soluble dietary fiber from peanut null. D101 type resin as the optimal absorbent material for soluble dietary fiber from peanut null was decided and its dynamic absorbing behavior was studied. The results showed that:D101 type resin possesses higher absorption and desorption capacity;the optimal technological conditions of adsorption velocity was 2 ml/min,solution concentration was 1 mg/mL to 2 mg/mL,solution pH was about 10,and the eluting solvent was 70%ethanol at 1 ml/min flow rate. UV spectral analysis and purity determination indicated the purified soluble dietary fiber from peanut null had high purity(96.4%)and contained little impurity(0.23%).
