粮食与油脂
糧食與油脂
양식여유지
CEREALS & OILS
2013年
2期
16-18
,共3页
核桃蛋白%ACE 抑制肽%功能性肽
覈桃蛋白%ACE 抑製肽%功能性肽
핵도단백%ACE 억제태%공능성태
walnut protein%ACE inhibitory peptied%function peptide
核桃 ACE 抑制肽经超滤后,其 ACE 抑制率由76.58%增至78.43%,再经 DA201–C 大孔吸附树脂脱盐纯化后,脱盐率达到98.70%,ACE 抑制率升高至80.73%;采用 Sephadex G–15凝胶分离后,核桃 ACE 抑制肽被分为三个组分,其中活性最大的 G2组分,其 ACE 抑制率达83.10%,且 IC50为1.308 mg/mL,G2组分主要分布在500 Da~180 Da,系为2~4个氨基酸残基组成小肽.
覈桃 ACE 抑製肽經超濾後,其 ACE 抑製率由76.58%增至78.43%,再經 DA201–C 大孔吸附樹脂脫鹽純化後,脫鹽率達到98.70%,ACE 抑製率升高至80.73%;採用 Sephadex G–15凝膠分離後,覈桃 ACE 抑製肽被分為三箇組分,其中活性最大的 G2組分,其 ACE 抑製率達83.10%,且 IC50為1.308 mg/mL,G2組分主要分佈在500 Da~180 Da,繫為2~4箇氨基痠殘基組成小肽.
핵도 ACE 억제태경초려후,기 ACE 억제솔유76.58%증지78.43%,재경 DA201–C 대공흡부수지탈염순화후,탈염솔체도98.70%,ACE 억제솔승고지80.73%;채용 Sephadex G–15응효분리후,핵도 ACE 억제태피분위삼개조분,기중활성최대적 G2조분,기 ACE 억제솔체83.10%,차 IC50위1.308 mg/mL,G2조분주요분포재500 Da~180 Da,계위2~4개안기산잔기조성소태.
The ACE inhibition rate of purified peptide from walnut protein was from 76.58% to 78.43%after ultrafiltration. It increased to 80.73% and the desaltion rate reached about 98.70% after deoxidation used macroporous resin of DA201–C,Sephadex G–15 gel was used to purify ACE inhibitory peptides to obtain a fraction G2 with IC50 of 1.308 mg/mL. The molecular mass of HPLC–purified walnut ACE inhibitory peptides was the range of 500 Da~180 Da. Results indicated that ultrafiltration,macroporous resin and gel filtration chromatography could better purify ACE inhibitory peptides from walnut.
