CAJ | 학술논문

以0?C 低温处理12 h 的高山离子芥幼苗cDNA 为检测子,以正常生长温度下的高山离子芥幼苗cDNA为驱动子,应用抑制性消减杂交技术,构建冰缘植物高山离子芥的消减文库,筛选在低温下高表达的基因片段.随机挑取96个阳性克隆进行测序分析,得到39个独立的基因片段,26个和已知基因有较高的同源性,13个为未知基因.基因分析表明:高山离子芥在低温胁迫下,微丝交联蛋白Actin cross linking protein很可能在感受低温的过程中行使作用,而DREB2A和ABF1协同作用一起调节下游冷响应基因的表达,为进一步研究高山离子芥的抗冻机制奠定了基础.
이0?C 저온처리12 h 적고산리자개유묘cDNA 위검측자,이정상생장온도하적고산리자개유묘cDNA위구동자,응용억제성소감잡교기술,구건빙연식물고산리자개적소감문고,사선재저온하고표체적기인편단.수궤도취96개양성극륭진행측서분석,득도39개독립적기인편단,26개화이지기인유교고적동원성,13개위미지기인.기인분석표명:고산리자개재저온협박하,미사교련단백Actin cross linking protein흔가능재감수저온적과정중행사작용,이DREB2A화ABF1협동작용일기조절하유랭향응기인적표체,위진일보연구고산리자개적항동궤제전정료기출.
Suppression subtractive hybridization (SSH) was applied to identify genes involved in cold stress in an alpine subnival plant Chorispora bungeana, taking Chorispora bungeana seedlings under 0?C for 12 h as the tester and untreated seedlings as the driver and and the differentially expressed genes library was constructed. A total of 96 positive clones were sequenced, and 39 unigenes were identified and analyzed, with the result that 26 showed a high identity with known genes while 13 had no matches in the GeneBank and were listed as unknown genes. Gene function analysis showed that an actin cross the linking protein (F1) might play a role during the cold sensing while DREB2A and ABF1 might work together to regulate the downstream cold responsive genes. These results can lay a foundation for further study of Chorispora bungeana cold tolerance mechanisms.