兰州大学学报(自然科学版)
蘭州大學學報(自然科學版)
란주대학학보(자연과학판)
JOURNAL OF LANZHOU UNIVERSITY
2013年
1期
92-99
,共8页
张腾国%张艳%夏小慧%王娟%王圆圆%王宁%孙万仓
張騰國%張豔%夏小慧%王娟%王圓圓%王寧%孫萬倉
장등국%장염%하소혜%왕연%왕원원%왕저%손만창
低温胁迫%油菜%MAPKK抑制剂%抗氧化酶
低溫脅迫%油菜%MAPKK抑製劑%抗氧化酶
저온협박%유채%MAPKK억제제%항양화매
low temperature stress%Brassica napus%MAPKK inhibitor%anti-oxidative enzyme
以两种油菜品种天油2号和陇油6号作为供试材料,研究了MAPKK抑制剂U0126对低温胁迫油菜幼苗叶绿素、Fv/Fm、抗氧化酶活性的影响.结果表明:低温胁迫下,油菜幼苗叶绿素质量分数、Fv/Fm下降, MDA质量分数增加, CAT, APX, GR, SOD活性先增加后下降. MAPKK抑制剂U0126预处理再低温胁迫后,与单独低温处理相比,叶绿素质量分数、Fv/Fm降低, MDA质量分数增加,抗氧化酶活性均下降.表明MAP激酶级联途径参与油菜抗低温胁迫转导途径,缓解低温胁迫造成的氧化损伤,对其抗氧化损伤起了一定正调控作用.
以兩種油菜品種天油2號和隴油6號作為供試材料,研究瞭MAPKK抑製劑U0126對低溫脅迫油菜幼苗葉綠素、Fv/Fm、抗氧化酶活性的影響.結果錶明:低溫脅迫下,油菜幼苗葉綠素質量分數、Fv/Fm下降, MDA質量分數增加, CAT, APX, GR, SOD活性先增加後下降. MAPKK抑製劑U0126預處理再低溫脅迫後,與單獨低溫處理相比,葉綠素質量分數、Fv/Fm降低, MDA質量分數增加,抗氧化酶活性均下降.錶明MAP激酶級聯途徑參與油菜抗低溫脅迫轉導途徑,緩解低溫脅迫造成的氧化損傷,對其抗氧化損傷起瞭一定正調控作用.
이량충유채품충천유2호화롱유6호작위공시재료,연구료MAPKK억제제U0126대저온협박유채유묘협록소、Fv/Fm、항양화매활성적영향.결과표명:저온협박하,유채유묘협록소질량분수、Fv/Fm하강, MDA질량분수증가, CAT, APX, GR, SOD활성선증가후하강. MAPKK억제제U0126예처리재저온협박후,여단독저온처리상비,협록소질량분수、Fv/Fm강저, MDA질량분수증가,항양화매활성균하강.표명MAP격매급련도경삼여유채항저온협박전도도경,완해저온협박조성적양화손상,대기항양화손상기료일정정조공작용.
@@@@An experiment on Brassica napu seedings (Tianyou 2 and Longyou 6) was conducted to investigate the effects of MAPKK inhibitors on the chlorophyll content, maximum rate of photochemistry(Fv/Fm), and various antioxidant enzyme activities of Brassica napus seedings under low temperature stress. The results show that, in Brassica napu seedings under low temperature stress the chlorophyll content and the maximum rate of photochemistry were reduced, the content of MDA increased, while the activities of CAT, APX, GR, SOD increased first but decreased afterwards. Compared with low temperature treatment alone, MAPKK inhibitors U0126 pretreatment decreased the chlorophyll content and the maximum rate of photochemistry, as well as various antioxidant enzyme activities, while MDA content increased. In conclusion, MAPK cascades take part in the transduction pathways of low temperature stress and play a positive role in antioxidative damage.