CAJ | 학술논문

细菌群落在养殖发酵床中担负着重要的调节与废物发酵转化作用.直接从不同发酵床层次样品中提取细菌总 DNA,构建发酵床不同层次垫料的细菌基因克隆文库.试验表明,发酵床表层45株细菌中未培养微生物占39.5%,剩余细菌分属13个不同种,优势菌不明显;中层发酵床垫料45株细菌中未培养微生物占15.5%,剩余细菌分属10个不同种,优势细菌为Rhodanobacter sp.和Frateuria sp.;深层发酵床垫料中检测到的44株细菌不含未培养微生物,该层细菌主要有14个不同的种,优势细菌为 Rhodanobacter sp.占47.7%,其余种属相对较少且数量平均,表明发酵床垫料细菌呈明显的多样性.
세균군락재양식발효상중담부착중요적조절여폐물발효전화작용.직접종불동발효상층차양품중제취세균총 DNA,구건발효상불동층차점료적세균기인극륭문고.시험표명,발효상표층45주세균중미배양미생물점39.5%,잉여세균분속13개불동충,우세균불명현;중층발효상점료45주세균중미배양미생물점15.5%,잉여세균분속10개불동충,우세세균위Rhodanobacter sp.화Frateuria sp.;심층발효상점료중검측도적44주세균불함미배양미생물,해층세균주요유14개불동적충,우세세균위 Rhodanobacter sp.점47.7%,기여충속상대교소차수량평균,표명발효상점료세균정명현적다양성.
Microorganism has an important regulatory and bioconversion function in fermentation bed of pig-breeding. The bacterial DNA samples are directly extracted from different depth in fermentation bed. The bacterial gene clone library is constructed correspondingly for these different types of samples. The results show that the uncultured microorganism appeared in the surface of the fermentation bed, which accounts for 39.5%of the total 45 strains detected. The others belong to 13 different species of bacteria and no predominant bacteria detected. In the middle layer of fermentation bed, uncultured microorganism accounts for 15.5%of the total 45 strains detected. The others consist of 10 different species of bacteria. The predominant bacteria are Rhodanobacter sp. and Frateuria sp. respectively. The uncultured microorganism is not detected in all 44 bacteria strains in the bottom of fermentation bed. There are 14 different bacteria genus in this layer. The predominant bacteria is Rhodanobacter sp., accounting for 47.7%in all the bacteria detected. The others are in a less proportion of total microbial population in this layer. The results demonstrate that the fermented bed in pig-farming manifested an obvious bacterial diversity.