CAJ | 학술논문

目的探讨不同消毒时间和消毒次数对安瓿内注射液微生物存活量的影响.方法取400支10 mL 0.9%氯化钠的注射用安瓿,随机分为实验组和对照组各200支,实验组安瓿分别在砂轮划痕前后用酒精棉签各擦拭1次,对照组安瓿仅在划痕后用酒精棉签擦拭1次,每支安瓿用注射器抽取药液进行琼脂培养.另取1200支安瓿,随机分为A、B、C、D、E、F共6组各200支,A、B、C 3组于洁净台中进行与对照组相同的操作,但A组擦拭1s,B组擦拭2 s、C组擦拭5 s,而 D、E、F 3组则于治疗室中分别进行与A、B、C 3组相同的操作.结果安瓿开启前进行1次消毒与进行2次消毒的药液微生物培养结果差异无统计学意义(P>0.05);在洁净台或治疗室内操作,安瓿开启前不同擦拭时间药液的微生物培养结果差异均无统计学意义(P>0.05).结论增加安瓿开启前的消毒次数、擦拭时间无法显著地降低安瓿内药液的微生物污染情况.
목적탐토불동소독시간화소독차수대안부내주사액미생물존활량적영향.방법취400지10 mL 0.9%록화납적주사용안부,수궤분위실험조화대조조각200지,실험조안부분별재사륜화흔전후용주정면첨각찰식1차,대조조안부부재화흔후용주정면첨찰식1차,매지안부용주사기추취약액진행경지배양.령취1200지안부,수궤분위A、B、C、D、E、F공6조각200지,A、B、C 3조우길정태중진행여대조조상동적조작,단A조찰식1s,B조찰식2 s、C조찰식5 s,이 D、E、F 3조칙우치료실중분별진행여A、B、C 3조상동적조작.결과안부개계전진행1차소독여진행2차소독적약액미생물배양결과차이무통계학의의(P>0.05);재길정태혹치료실내조작,안부개계전불동찰식시간약액적미생물배양결과차이균무통계학의의(P>0.05).결론증가안부개계전적소독차수、찰식시간무법현저지강저안부내약액적미생물오염정황.
Objective To observe the impact of different disinfection time and disinfection times of ampoule on the survival amount of microorganism. Methods A total of 400 tube sodium chloride injection ampoules (specification: 0.9% 10 mL) were randomly divided into experimental group and control group. Each in experimental group was wiped around with alcohol swabs before and after the wheel scratch while those in control group were only wiped after the wheel scratch with alcohol swabs. Then the liquid of each ampoule was extracted with syringe and cultured on agar. Another 1 200 ampoules were randomly divided into group A, B, C, D, E, F equally. The same operation performed in control group was conducted in group A, B, C in the clean bench, however, in group A, the wiping lasted for only 1 s, in group B 2 s, and in group C, 5 s. In group D, E, F, the same operation performed in Group A, B, C was performed but in the treatment room. Results The result of the liquid microbial culture indicated that there was no significant difference whether disinfecting once or twice before the opening of the ampoule (P>0.05). In the clean bench or in the treatment room, the effect of different wipe time before opening the ampoule showed no significant difference (P>0.05). Conclusion Increased disinfection wipe time (circles) or disinfection wipe times before the opening of ampoule can not significantly reduce the microbial contamination of the liquid in the ampoule.