CAJ | 학술논문

为了高值化利用条斑紫菜资源,该文采用交替双频逆流超声辅助提取技术生产条斑紫菜蛋白和多糖混合产品.研究了复合双频和交替双频2种超声模式对条斑紫菜蛋白和多糖提取效果的影响,采用中心组合设计响应面优化试验进行工艺优化.结果显示,交替双频超声模式显著优于复合双频超声模式.料液比、提取时间和温度均对提取效果影响显著.确定的交替双频逆流超声辅助提取的最优条件为:料液比14 mg/mL、时间88 min、温度41℃、pH 值9.0、15和20 kHz 交替双频超声、超声交替工作时间3 s、超声功率200 W/L.在此最优条件下,条斑紫菜蛋白和多糖混合产品的生产能力最高,单位质量原料生产出的产品得率为(48.18?0.08)%,其中蛋白得率为(24.35?0.07)%,纯度为(45.61?0.33)%,多糖得率为(23.83?0.02)%,纯度为(44.64?0.37)%,比传统提取方法,得率提高了168%,时间缩短了64%;比单频逆流超声辅助提取方法,得率提高了50%,时间缩短了18%.从生产成本分析,交替双频逆流超声辅助提取方法具有明显技术优势和工业推广价值.
위료고치화이용조반자채자원,해문채용교체쌍빈역류초성보조제취기술생산조반자채단백화다당혼합산품.연구료복합쌍빈화교체쌍빈2충초성모식대조반자채단백화다당제취효과적영향,채용중심조합설계향응면우화시험진행공예우화.결과현시,교체쌍빈초성모식현저우우복합쌍빈초성모식.료액비、제취시간화온도균대제취효과영향현저.학정적교체쌍빈역류초성보조제취적최우조건위:료액비14 mg/mL、시간88 min、온도41℃、pH 치9.0、15화20 kHz 교체쌍빈초성、초성교체공작시간3 s、초성공솔200 W/L.재차최우조건하,조반자채단백화다당혼합산품적생산능력최고,단위질량원료생산출적산품득솔위(48.18?0.08)%,기중단백득솔위(24.35?0.07)%,순도위(45.61?0.33)%,다당득솔위(23.83?0.02)%,순도위(44.64?0.37)%,비전통제취방법,득솔제고료168%,시간축단료64%;비단빈역류초성보조제취방법,득솔제고료50%,시간축단료18%.종생산성본분석,교체쌍빈역류초성보조제취방법구유명현기술우세화공업추엄개치.
Porphyra yezoensis is rich in high contents of protein and polysaccharide, while that have not been fully used and developed. In order to improve resource utilization rate of Porphyra yezoensis, a new technology of alternating two-frequency countercurrent ultrasonic-assisted extraction is developed to produce high-yield protein and polysaccharide mixed products. The effects of two ultrasonic ways were investigated in this research: the composite working and the alternating working of two-frequency (two-frequency of A: 15 and B: 20 kHz, A: 15 and C: 28 kHz, and B: 20 and C: 28 kHz, respectively referred to as the AB composition, AB alternation, AC composition, AC alternation, BC composition, and BC alternation), on the product yield (expressed as the total yields of protein and polysaccharide). The response surface optimization tests for three key factors of material to water ratio, extraction time, and temperature were conducted to optimize the extraction process, based on the central composite design (CCD). Moreover, in order to analyze the feasibility of alternating two-frequency countercurrent ultrasonic-assisted extraction technique, the extraction performance of the alternating two-frequency countercurrent ultrasonic-assisted extraction method was compared with those of the mono-frequency countercurrent ultrasonic-assisted extraction and the traditional extraction methods. The results showed that the alternating ultrasonic way was significantly better than the composite one and the alternating two-frequency of 15 and 20 kHz was the most effective. The factors: material to water ratio, extraction time, and temperature had significant effects on the extraction process. Therefore, the established optimum conditions of alternating two-frequency countercurrent ultrasonic-assisted extraction were as follows: the material to water ratio was 14 mg/mL, the extraction time was 88 min, the temperature was 41oC, the pH value was 9.0, the ultrasonic power was 200 W/L, the alternating two-frequency was 15 and 20 kHz, and an alternating working time of 3 s. Under these conditions, the production capacity of protein and polysaccharide mixed products from Porphyra yezoensis achieved the highest with the product yield of (48.18?0.08)%, in which the protein yield was (24.35?0.07)%, the corresponding protein purity was (45.61?0.33)%, the polysaccharide yield was (23.83?0.02)%, and the corresponding polysaccharide purity was (44.64?0.37)%, respectively. The product yield was increased by 168% and the extraction time was decreased by 64% compared with the traditional extraction method. In addition, the product yield was increased by 50% and the extraction time was reduced by 18% compared with the mono-frequency countercurrent ultrasonic-assisted extraction. In terms of production cost, the alternating two-frequency countercurrent ultrasonic-assisted extraction is technically advantageous and is worth being promoted in the industry compared with the mono-frequency countercurrent ultrasonic-assisted extraction and the traditional extraction methods.