CAJ | 학술논문

通过对影响高压CO2杀菌技术(HPCD)的主要因素温度、压力、保压时间进行分析,利用正交试验,以杀灭猪肉糜中枯草芽孢的致死率为指标,对猪肉糜中枯草芽孢的杀菌条件进行优化,并优选出辅助试剂来协同HPCD进行灭菌,在此基础上,推测HPCD致死枯草芽孢的机理.结果表明:HPCD能有效杀灭枯草芽孢,3个因素对杀菌效果的影响程度为压力>保压时间>温度,最优参数组合为30MPa、40℃、保压60min,致死率可以达到3.39.添加辅助试剂的最佳杀菌方式为:既添加芽孢的萌发剂又添加天然抑菌剂,致死率可达5.16.单独添加萌发剂的效果要好于单独添加天然抑菌剂的,故猜测HPCD致死芽孢的机理为先促使芽孢萌发而后将其营养体杀死.
통과대영향고압CO2살균기술(HPCD)적주요인소온도、압력、보압시간진행분석,이용정교시험,이살멸저육미중고초아포적치사솔위지표,대저육미중고초아포적살균조건진행우화,병우선출보조시제래협동HPCD진행멸균,재차기출상,추측HPCD치사고초아포적궤리.결과표명:HPCD능유효살멸고초아포,3개인소대살균효과적영향정도위압력>보압시간>온도,최우삼수조합위30MPa、40℃、보압60min,치사솔가이체도3.39.첨가보조시제적최가살균방식위:기첨가아포적맹발제우첨가천연억균제,치사솔가체5.16.단독첨가맹발제적효과요호우단독첨가천연억균제적,고시측HPCD치사아포적궤리위선촉사아포맹발이후장기영양체살사.
The effects of experimental conditions including temperature, pressure and dwell time on the efficacy of high pressure carbon dioxide (HPCD) treatment for inactivating Bacillus subtilis in pork were examined in this study. An orthogonal array design was used to optimize the operating conditions based on bacterial lethality. Besides, optimal chemical additives for enhancing the bactericidal effect of HPCD treatment against Bacillus subtilis were screened and the bactericidal mechanism of HPCD treatment was analyzed. HPCD was effective in inactivating Bacillus subtilis. The order of effect of the HPCD treatment conditions on bacterial lethality, listed in decreasing order, was as follows:pressure, dwell time and temperature, and their optimized values were 30 MPa, 60 min and 40℃, respectively, resulting in a lethal rate as high as 3.39. The simultaneous addition of inhibitor and inducer was the best way to enhance the inactivation of Bacillus subtilis by HPCD and the resulting lethal rate was as high as 5.16;moreover, better bactericidal effect was observed for inducer alone compared with inhibitor alone. Hence, we speculated that the bactericidal mechanism of HPCD against Bacillus subtilis may be achieved by first promoting spore germination and then killing vegetative cells.