CAJ | 학술논문

要:通过研究得出,在聚合电压U为0~1.6V、聚合段数为22、模板三聚氰胺(MEL)浓度为4mmol/L、单体甲基丙烯酸(MAA)浓度为10mmol/L、成膜剂邻苯二胺(o-PD)浓度为18mmol/L条件下,以pH 8.0的BR缓冲液作为溶剂和电解液,用电聚合方法,直接在石英晶体微天平(QCM)传感器电极上修饰的分子印迹薄膜,其对目标分子MEL具有良好的频率表征效果,频移值为59Hz.通过相同3个修饰电极的频率表征变化来看,电极对目标分子的识别表征极不稳定.这与手动进样方式、环境较大噪声和电极本身差异密切相关,难以建立对目标分子的标准曲线(r=0.966)实现准确定量分析.
요:통과연구득출,재취합전압U위0~1.6V、취합단수위22、모판삼취청알(MEL)농도위4mmol/L、단체갑기병희산(MAA)농도위10mmol/L、성막제린분이알(o-PD)농도위18mmol/L조건하,이pH 8.0적BR완충액작위용제화전해액,용전취합방법,직접재석영정체미천평(QCM)전감기전겁상수식적분자인적박막,기대목표분자MEL구유량호적빈솔표정효과,빈이치위59Hz.통과상동3개수식전겁적빈솔표정변화래간,전겁대목표분자적식별표정겁불은정.저여수동진양방식、배경교대조성화전겁본신차이밀절상관,난이건립대목표분자적표준곡선(r=0.966)실현준학정량분석.
Molecularly imprinted membrane for direct modification of QCM sensor electrode was prepared by electropolymerization using BR buffer (pH 8.0) as the solvent and the electrolyte solution under the conditions of 0–1.6 V, 22, 4 mmol/L, 10 mmol/L and 18 mmol/L for polymerization voltage, the number of polymeric segments, template (melamine, MEL) concentration, monomer (methacrylic acid, MAA) concentration and o-PD (film-forming agent) concentration, respectively, The prepared membrane had good performance for frequency characterization of MEL with frequency shift of 59 Hz. Three replicates of the modified QCM sensor electrode showed variations in frequency characterization, suggesting its very poor stability, which may be closely related to manual sampling, the high level of environmental noise and the electrode itself. As a result, it is hard to establish a standard curve for accurate quantification of melamine.