CAJ | 학술논문

利用CV1菌株液体发酵生产云芝多糖,分别考察培养基碳源、培养基氮源、碳源浓度、氮源浓度、无机盐对该菌株产云芝多糖性能的影响,采用正交实验法确立了云芝CV1产胞外多糖的最佳培养基:蔗糖:4%,大豆粉:5%;KH2PO4:0.1%;MgSO4:0.1%;在摇床转速:200r/min、发酵温度:26-28℃、发酵周期:65h、培养基初始pH值:自然条件下云芝CV1的胞外粗多糖(EPS)含量可达7.458g/L,菌丝体(Biomass)可达64.619g/L.
이용CV1균주액체발효생산운지다당,분별고찰배양기탄원、배양기담원、탄원농도、담원농도、무궤염대해균주산운지다당성능적영향,채용정교실험법학립료운지CV1산포외다당적최가배양기:자당:4%,대두분:5%;KH2PO4:0.1%;MgSO4:0.1%;재요상전속:200r/min、발효온도:26-28℃、발효주기:65h、배양기초시pH치:자연조건하운지CV1적포외조다당(EPS)함량가체7.458g/L,균사체(Biomass)가체64.619g/L.
Use CV1 strain liquid fermentation producing polysaccharide of the Coriolus versicolor , separately in-spected medium carbon source, medium nitrogen source, carbon source concentration and nitrogen source, inorganic salt concentration of the strains producing the effect on the properties of polysaccharides, using the orthogonal ex-periment method established CV1 produce extracellular polysaccharide the best medium: cane sugar 4%, soybean meal 5%, KH2PO4 0.1%, MgSO4 0.1%, 200rpm, 26-28℃, 65h, medium initial pH value: Natural conditions CV1 of extracellular polysaccharide (EPS) content can reach 7.458g/L, Biomass can reach 64.619g/L.