CAJ | 학술논문

本研究在对Spinigerinα抗菌肽成功的进行了克隆及表达的基础上,对Spinigerin α抗菌肽的理化性质,包括抑菌特性、温度、pH稳定性等外界条件对其抗菌活性的影响进行了进一步研究.研究结果表明:Spinigerin α抗菌肽的自身浓度、温度、及pH对其抑菌活性均有重要影响,对金黄色葡萄球菌、沙门氏菌、大肠杆菌的最小抑菌浓度分别为1.52%、3.13%、12.5%.当抗菌肽加热至60-100℃时,抗菌肽抑菌效果依然明显,当加热至121℃时,其抑菌效果开始有下降趋势.当处于pH值6.0-8.0的近中性环境中,Spinigerinα抗菌肽的抗菌活性最强.
본연구재대Spinigerinα항균태성공적진행료극륭급표체적기출상,대Spinigerin α항균태적이화성질,포괄억균특성、온도、pH은정성등외계조건대기항균활성적영향진행료진일보연구.연구결과표명:Spinigerin α항균태적자신농도、온도、급pH대기억균활성균유중요영향,대금황색포도구균、사문씨균、대장간균적최소억균농도분별위1.52%、3.13%、12.5%.당항균태가열지60-100℃시,항균태억균효과의연명현,당가열지121℃시,기억균효과개시유하강추세.당처우pH치6.0-8.0적근중성배경중,Spinigerinα항균태적항균활성최강.
Based on the successful cloning and expression of Spinigerin antimicrobial peptide gene, this paper con-ducts a research on the effects of external conditions of antibacterial characteristics, temperature, pH stability of Spinigerin peptide on its antibacterial activity. Results show that: the concentration of Spinigerin α, temperature, pH are all have important impact on its bacteriostatic activity, the smallest antibacterial activities on Staphylococcus aureus, Escherichia coli, Salmonella are 1.52%, 3.13% and 12.5% respectively; when Spinigerin α is heated to 60-100℃, the bacteriostatic effect is obvious, while heated to 121℃, the effect begins to decline; when the pH is nearly neutral environment (6.0-8.0), Spinigerin antibacterial peptides have the strongest antibacterial activity.