CAJ | 학술논문

目的通过检测一种新型乳糖酶在不同条件下对牛乳中乳糖的水解率,研究其在制备低乳糖牛奶的最适条件.方法产乳糖酶基因工程菌株发酵并提取乳糖酶,分别在10,25和37℃条件下按不同比例添加该新型乳糖酶液,使用HPLC检测其乳糖水解率.结果该酶在10℃(0.9%)水解率可达83%以上,25℃(0.9%)水解率93%以上,37℃(0.3%)水解率94%以上.结论该酶在低温下具有良好的乳糖水解率,在工业上具有广阔的应用前景.
목적통과검측일충신형유당매재불동조건하대우유중유당적수해솔,연구기재제비저유당우내적최괄조건.방법산유당매기인공정균주발효병제취유당매,분별재10,25화37℃조건하안불동비례첨가해신형유당매액,사용HPLC검측기유당수해솔.결과해매재10℃(0.9%)수해솔가체83%이상,25℃(0.9%)수해솔93%이상,37℃(0.3%)수해솔94%이상.결론해매재저온하구유량호적유당수해솔,재공업상구유엄활적응용전경.
Objective To study the application of a new lactase in the production of low-lactose milk through the investigate of hydrolysis rate under different conditions. Methods The hydrolysis of lactose was detected by HPLC. The lactase was added into milk with different ratio. Then the mixture was treated at 10 ℃、25 ℃ and 37 ℃ using water bar. Results The hydrolysis rate reached a value which was more than 83% with the ratio of 0.9% at 10℃, more than 93% with the ratio of 0.9% at 25℃, and more than 94%, with the ratio of 0.3% at 37℃. Conclusion The new lactase was suitable for production of low-lactose milk at low temperatures, and it has broad application prospects in the industry.