CAJ | 학술논문

目的通过响应面法,优化猪脾中免疫活性多肽的提取工艺.方法选择适当的酶水解猪脾脏,并在单因素试验的基础上,采用Box-Behnken设计优化提取工艺,建立各因素与提取液中多肽含量的数学模型,最后采用淋巴细胞转化试验初步验证其活性.结果获得了猪脾中免疫活性多肽提取工艺:料液比1:2.6 g/mL,温度40℃,pH 7.0,胰蛋白酶浓度485 U/g,中性蛋白酶浓度1711 U/g,每100 g含活性多肽2.930 g,与理论计算值2.929 g基本一致,活性约为对照组的1.5倍.结论确定了猪脾免疫活性多肽最佳提取工艺.
목적통과향응면법,우화저비중면역활성다태적제취공예.방법선택괄당적매수해저비장,병재단인소시험적기출상,채용Box-Behnken설계우화제취공예,건립각인소여제취액중다태함량적수학모형,최후채용림파세포전화시험초보험증기활성.결과획득료저비중면역활성다태제취공예:료액비1:2.6 g/mL,온도40℃,pH 7.0,이단백매농도485 U/g,중성단백매농도1711 U/g,매100 g함활성다태2.930 g,여이론계산치2.929 g기본일치,활성약위대조조적1.5배.결론학정료저비면역활성다태최가제취공예.
Objective To optimize pig spleen immunity activity peptides extraction process by response surface method. Methods Choose appropriate enzymes for hydrolysis of pig spleen, using the design of Box-Behnken to optimize extraction process on the basis of single factor test, and establish a mathematical model of all factors for the content of peptides. Finally, a lymphocyte transformation test was conducted for the preliminary validation of its activity. Results The best extraction process was the proportion of material and liquid 1:2.6 g/mL, temperature: 40 ℃, pH 7.0, concentration of trypsin 485 U/g, concentration of neutral protease 1711 U/g. The content of active peptides was 2.930 g/100g, basically the same level of theoretical value 2.929g /100g. Activity was about 0.5 times higher than the control group. Conclusion The optimum extraction process of pig spleen immunity activity peptides was determined as the proportion of material and liquid 1:2.6 g/mL, temperature 40 ℃, pH 7.0, concentration of trypsin 485 U/g, concentration of neutral protease 1711 U/g.