CAJ | 학술논문

目的研究利用固相提取净化、高效液相色谱-电喷雾串联三重四级杆质谱检测分析水中微囊藻毒素MC-LR和MC-RR.方法水样品经玻璃纤维滤纸过滤后,经C18固相提取柱富集净化,氮吹后定容.流动相中添加0.1%甲酸,采用梯度洗脱模式,经C18反相色谱柱分离,以电喷雾串联三重四级杆质谱定性、定量测定.结果线性定量范围0.1~5.0μg/kg,检测低限为MC-LR 0.01μg/kg,MC-RR 0.02μg/kg.结论此方法操作简便、定性定量能力较强,可作为检测水质量和评估食品安全风险的可靠方法.
목적연구이용고상제취정화、고효액상색보-전분무천련삼중사급간질보검측분석수중미낭조독소MC-LR화MC-RR.방법수양품경파리섬유려지과려후,경C18고상제취주부집정화,담취후정용.류동상중첨가0.1%갑산,채용제도세탈모식,경C18반상색보주분리,이전분무천련삼중사급간질보정성、정량측정.결과선성정량범위0.1~5.0μg/kg,검측저한위MC-LR 0.01μg/kg,MC-RR 0.02μg/kg.결론차방법조작간편、정성정량능력교강,가작위검측수질량화평고식품안전풍험적가고방법.
Objective To detect microcystin-LR and microcystin-RR in water by HPLC-MS/MS. Methods The samples were filtered through the glass fiber filter, and purified by C18 solid phase extraction column, then concentrated through N2 blowing. With adding 0.1 %(v/v) formic acid to the mobile phase, chromatographic separation was performed on a C18 reverse column by gradient elution mode, and the analyte was determined by HPLC-MS/MS. Results The linear determination range was 0.1-5.0 μg/kg, and the detection limit was 0.01 μg/kg for microcystin-LR,0.02 μg/kg for microcystin-RR. Conclusion The method can be easy to operate, and suitable for water sample detection and food safety risk assessment.