饲料工业
飼料工業
사료공업
FEED INDUSTRY
2013年
4期
35-38
,共4页
樊振%买尔哈巴·艾合买提%美合热阿依·木台力甫%乌斯满·依米提
樊振%買爾哈巴·艾閤買提%美閤熱阿依·木檯力甫%烏斯滿·依米提
번진%매이합파·애합매제%미합열아의·목태력보%오사만·의미제
青贮饲料%乳酸菌%冷冻干燥%保护剂
青貯飼料%乳痠菌%冷凍榦燥%保護劑
청저사료%유산균%냉동간조%보호제
silage%Lactic acid bacteria%freeze drying%cryoprotectants
通过离心条件的选择,获得最大浓度的乳酸菌,进而利用正交试验设计,确定适合植物乳杆菌和肠膜状明串株葡聚糖亚种的最佳保护剂配方.试验结果表明,两株菌均在5000 r/min的条件下离心10 min,所得的活菌数最多.试验确定植物乳杆菌的最佳保护剂配方为:脱脂乳15%、海藻糖6%、甘油2%、抗坏血酸3%;肠膜状明串株葡聚糖亚种的最佳保护剂配方为:脱脂乳15%、海藻糖6%、甘油1%、抗坏血酸2%.
通過離心條件的選擇,穫得最大濃度的乳痠菌,進而利用正交試驗設計,確定適閤植物乳桿菌和腸膜狀明串株葡聚糖亞種的最佳保護劑配方.試驗結果錶明,兩株菌均在5000 r/min的條件下離心10 min,所得的活菌數最多.試驗確定植物乳桿菌的最佳保護劑配方為:脫脂乳15%、海藻糖6%、甘油2%、抗壞血痠3%;腸膜狀明串株葡聚糖亞種的最佳保護劑配方為:脫脂乳15%、海藻糖6%、甘油1%、抗壞血痠2%.
통과리심조건적선택,획득최대농도적유산균,진이이용정교시험설계,학정괄합식물유간균화장막상명천주포취당아충적최가보호제배방.시험결과표명,량주균균재5000 r/min적조건하리심10 min,소득적활균수최다.시험학정식물유간균적최가보호제배방위:탈지유15%、해조당6%、감유2%、항배혈산3%;장막상명천주포취당아충적최가보호제배방위:탈지유15%、해조당6%、감유1%、항배혈산2%.
The effects of centrifugation condition on livability of Lactobacillus plantarum and Leuconostoc dextran subspecies were studied in this research. The result manifested that the most optimum condition parameter for preparing the two suspension were 10 min, 5 000 r/min. The optimum cryoprotectants formula for Lactobacillus plantarum was determined by the orthogo?nal trial as: nonfat milk powder 15%, trehalose 6%, glycerol 2%, ascorbic acid 3%; the opti?mum cryoprotectants formula for Leuconostoc dextran was determined as: nonfat milk powder 15%, trehalose 6%, glycerol 1%, ascorbic acid 2%.