CAJ | 학술논문

研究旨在筛选高产脂肪酶活性的菌株,并鉴定其菌属及确定其最佳产酶条件.采集富含油脂的土壤,通过溴甲酚紫平板法进行菌株分离筛选,并采用橄榄油乳化法测定脂肪酶活力.利用真菌ITS序列通用引物进行PCR扩增,将测序结果Blast比对,并建系统发育树.采用正交试验设计优化产酶条件.通过筛选得到1株产脂肪酶的真菌菌株GW-1,经鉴定为黑曲霉Aspergillus niger.经单因素试验和正交试验得到该菌的最佳产酶条件为:葡萄糖0.5%、黄豆粉2.0%、橄榄油1.0%、MgSO4·7H2O 0.1%、pH值9.0、接种量8%、装液量50 ml/250 ml、发酵4 d.在此发酵条件下,其产酶活力可达39.82 U/(ml·min).试验结果显示,访菌株在产脂肪酶能力上具有显著优越性,且菌株GW-1产酶活力较优化前高出19.96 U/(ml·min),提高了100.50%.
연구지재사선고산지방매활성적균주,병감정기균속급학정기최가산매조건.채집부함유지적토양,통과추갑분자평판법진행균주분리사선,병채용감람유유화법측정지방매활력.이용진균ITS서렬통용인물진행PCR확증,장측서결과Blast비대,병건계통발육수.채용정교시험설계우화산매조건.통과사선득도1주산지방매적진균균주GW-1,경감정위흑곡매Aspergillus niger.경단인소시험화정교시험득도해균적최가산매조건위:포도당0.5%、황두분2.0%、감람유1.0%、MgSO4·7H2O 0.1%、pH치9.0、접충량8%、장액량50 ml/250 ml、발효4 d.재차발효조건하,기산매활력가체39.82 U/(ml·min).시험결과현시,방균주재산지방매능력상구유현저우월성,차균주GW-1산매활력교우화전고출19.96 U/(ml·min),제고료100.50%.
This experiment was conducted to screen high lipase activity strains, identify the bac?terium and define the optimum fermentation conditions. The strain was screened from collected greasy soil through bromocresol purple plate method and the lipase activity was determined with olive oil emulsification method. PCR was performed using the Fungi on ITS sequence universal primers. The sequencing results was Blasted and the development tree was installed. The opti?mum enzyme production conditions were designed using orthogonal test. A Fungi strain GW-1 producing lipase was screened, which was identified as Aspergillus niger. The optimum fermenta?tion conditions for lipase production were got from the single factor experiments and the orthogo?nal experiments by strain GW-1 were 0.5%glucose, 2.0%soybean powder, 1.0%olive oil, 0.1%MgSO4·7H2O, initial pH 9.0, 8%inoculums volume, and bottle filling capacity 50 ml/250 ml, fer?mentation time 4 d. Under this optimum condition, the lipase activity of strain GW-1 reached 39.82 U/(ml·min). These results indicated that the enzyme activity produced by strain GW-1 was increased by 19.96 U/(ml·min) through optimization than before, up 100.50%.