CAJ | 학술논문

试验研究一株凝结芽孢杆菌BCRC11592的耐高温、耐酸、耐胆盐及对革兰氏阴性菌抑制的能力,并分析培养时间、温度、初始pH值、接种量和摇床转速等因素对菌体生长和产孢率的影响.结果证实该菌株芽孢有良好的抗逆性,具备开发为微生态饲料制剂的潜力.经优化后其最佳培养条件为培养时间24 h、温度40℃、初始 pH 值6.5、接种量106 cfu、摇床转数140 r/min,在此条件下菌液活菌数为3.5×108 cfu/ml,芽孢数为3.0×108 cfu/ml,产孢率为85.7%.
시험연구일주응결아포간균BCRC11592적내고온、내산、내담염급대혁란씨음성균억제적능력,병분석배양시간、온도、초시pH치、접충량화요상전속등인소대균체생장화산포솔적영향.결과증실해균주아포유량호적항역성,구비개발위미생태사료제제적잠력.경우화후기최가배양조건위배양시간24 h、온도40℃、초시 pH 치6.5、접충량106 cfu、요상전수140 r/min,재차조건하균액활균수위3.5×108 cfu/ml,아포수위3.0×108 cfu/ml,산포솔위85.7%.
This paper studied the stress resistance of the spores of Bacillus coagulans BCRC11592 to heat, acid, bile salt and bacteriostatic ability against Gram-negative bacteria, and investigated the influence on cell growth and sporulation rate of incubation time, tempera?ture, initial pH value, inoculum and rotation speed. The results confirmed the spores of this strain have good resistance for the potential application in probiotics and the optimal culture conditions were obtained by single-factor test test as follows: incubation time 24 h, temperature 40 ℃, initial pH 6.5, inoculum size 106 cfu, rotation speed 140 r/min. The number of viable cells reached 3.5×108 cfu/ml, the number of spores reached 3.0×108 cfu/ml, and the sporulation rate was 85.7%.