饲料工业
飼料工業
사료공업
FEED INDUSTRY
2013年
7期
52-54
,共3页
色氨酸%高效液相色谱法%饲料
色氨痠%高效液相色譜法%飼料
색안산%고효액상색보법%사료
tryptophan%HPLC%forage
试验采用高效液相色谱法测定饲料样品中色氨酸的含量.样品经过处理后,用Agilent C18(4.6 mm×150 mm,5μm)色谱柱分离样品,以0.0085 mol/l的乙酸钠+甲醇=95+5为流动相;检测波长280 nm,流动相流速1.0 ml/min.结果表明,色氨酸在4.656~298μg/ml范围内,峰面积与其浓度线性关系良好(r=0.9999),最低检测限为0.0335μg/ml,两种饲料中色氨酸含量均在国标允许的误差范围内.方法准确、简便、快速,适用于色氨酸的含量测定.
試驗採用高效液相色譜法測定飼料樣品中色氨痠的含量.樣品經過處理後,用Agilent C18(4.6 mm×150 mm,5μm)色譜柱分離樣品,以0.0085 mol/l的乙痠鈉+甲醇=95+5為流動相;檢測波長280 nm,流動相流速1.0 ml/min.結果錶明,色氨痠在4.656~298μg/ml範圍內,峰麵積與其濃度線性關繫良好(r=0.9999),最低檢測限為0.0335μg/ml,兩種飼料中色氨痠含量均在國標允許的誤差範圍內.方法準確、簡便、快速,適用于色氨痠的含量測定.
시험채용고효액상색보법측정사료양품중색안산적함량.양품경과처리후,용Agilent C18(4.6 mm×150 mm,5μm)색보주분리양품,이0.0085 mol/l적을산납+갑순=95+5위류동상;검측파장280 nm,류동상류속1.0 ml/min.결과표명,색안산재4.656~298μg/ml범위내,봉면적여기농도선성관계량호(r=0.9999),최저검측한위0.0335μg/ml,량충사료중색안산함량균재국표윤허적오차범위내.방법준학、간편、쾌속,괄용우색안산적함량측정.
The experiment was conducted to establish a HPLC for determination of tryptophan. Tryptophan was determinated on HPLC instrument fixed a Agilent C18 column(4.6 mm×150 mm, 5 μm) with UV detection at 280 nm, and the mobile phase was 0.008 5 mol/l sodium acetate so?lution+MeOH=95+5, the flow rate was 1.0 ml/min. The calibration curve was linear(r=0.999 9) within the range of 4.656~298 μg/ml for tryptophan, and LOD was 0.033 5 μg/ml,levels of tryp?tophan in the two feed were in reasonable range. This method was simple, quick, accurate and effective for determination of tryptophan. It was suitable for the quality control for tryptophan.
