食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2013年
1期
27-37
,共11页
祁志军*%寇立泉%王东方%刘爽
祁誌軍*%寇立泉%王東方%劉爽
기지군*%구립천%왕동방%류상
有机磷%多残留%抗原%免疫分析
有機燐%多殘留%抗原%免疫分析
유궤린%다잔류%항원%면역분석
organophosphorus%multiresidue%antigen%immunoassay
有机磷杀虫剂是一类重要的农业生产资料,在防治害虫和增加粮食产量方面发挥了极大的作用,但由于其大量使用带来的环境问题越来越受到人们的关注.因此,如何快速准确地检测其在农作物及环境中的残留、尤其是多种农药的残留,一直是研究的热点.近年来,基于抗原抗体特异性识别反应机理,研究者在农药多残留免疫分析方面做了大量的探索.本文针对有机磷类杀虫剂的多残留免疫分析,从采用混合抗体、多抗原决定簇抗体、共性结构抗体及单链可变区抗体几个方面做了详细地总结,并对存在的问题进行了探讨,以期为今后的研究提供借鉴.
有機燐殺蟲劑是一類重要的農業生產資料,在防治害蟲和增加糧食產量方麵髮揮瞭極大的作用,但由于其大量使用帶來的環境問題越來越受到人們的關註.因此,如何快速準確地檢測其在農作物及環境中的殘留、尤其是多種農藥的殘留,一直是研究的熱點.近年來,基于抗原抗體特異性識彆反應機理,研究者在農藥多殘留免疫分析方麵做瞭大量的探索.本文針對有機燐類殺蟲劑的多殘留免疫分析,從採用混閤抗體、多抗原決定簇抗體、共性結構抗體及單鏈可變區抗體幾箇方麵做瞭詳細地總結,併對存在的問題進行瞭探討,以期為今後的研究提供藉鑒.
유궤린살충제시일류중요적농업생산자료,재방치해충화증가양식산량방면발휘료겁대적작용,단유우기대량사용대래적배경문제월래월수도인문적관주.인차,여하쾌속준학지검측기재농작물급배경중적잔류、우기시다충농약적잔류,일직시연구적열점.근년래,기우항원항체특이성식별반응궤리,연구자재농약다잔류면역분석방면주료대량적탐색.본문침대유궤린류살충제적다잔류면역분석,종채용혼합항체、다항원결정족항체、공성결구항체급단련가변구항체궤개방면주료상세지총결,병대존재적문제진행료탐토,이기위금후적연구제공차감.
Organophosphorus pesticides (OPs), a kind of important agricultural production material, have been playing an important role in pest control and food production increase. But more and more attention has been paid on the serious environmental problems because of the excess use of OPs. The quick and accurate de-tection of residues, especially the multi-residues of OPs is always a research focus. In recent years, a great deal of research and exploration has been made on the multi-analyte immunoassay of pesticides based on the spe-cific recognition between antigen and antibody. This article reviewed the recent advances on the multi-residue determination of organophosphorus insecticides, including the progresses of mixed antibodies, antibodies against multi-antigenic determinants, antibodies against common moiety and single chain fragment variable (ScFv) antibodies, and discussed the existed problems and future research.