食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2013年
1期
61-70
,共10页
张璐%邱瑾%程燕%赵丽敏%吴中波%李梦琦%刘晓宇*
張璐%邱瑾%程燕%趙麗敏%吳中波%李夢琦%劉曉宇*
장로%구근%정연%조려민%오중파%리몽기%류효우*
毒死蜱%分子印迹%制备%识别机制
毒死蜱%分子印跡%製備%識彆機製
독사비%분자인적%제비%식별궤제
chlorpyrifos%molecular imprinting%preparation%recognition mechanism
目的制备能特异性识别农药毒死蜱的分子印迹聚合物并研究其识别机制,用于农作物中残留农药毒死蜱的分离富集及特异识别.方法利用沉淀法制备两种毒死蜱印迹聚合物,采用光谱手段、吸附实验和Scatchard 分析等研究其结合机制.结果制备的2种印迹聚合物对毒死蜱有特异吸附,以甲基丙烯酸和2-乙烯基吡啶为单体制备的 MIP存在两种结合位点.结论两种单体 MAA、2-VP分别与毒死蜱以1∶3和1∶2配比结合,两种聚合物均可用于样品中毒死蜱的分离富集.
目的製備能特異性識彆農藥毒死蜱的分子印跡聚閤物併研究其識彆機製,用于農作物中殘留農藥毒死蜱的分離富集及特異識彆.方法利用沉澱法製備兩種毒死蜱印跡聚閤物,採用光譜手段、吸附實驗和Scatchard 分析等研究其結閤機製.結果製備的2種印跡聚閤物對毒死蜱有特異吸附,以甲基丙烯痠和2-乙烯基吡啶為單體製備的 MIP存在兩種結閤位點.結論兩種單體 MAA、2-VP分彆與毒死蜱以1∶3和1∶2配比結閤,兩種聚閤物均可用于樣品中毒死蜱的分離富集.
목적제비능특이성식별농약독사비적분자인적취합물병연구기식별궤제,용우농작물중잔류농약독사비적분리부집급특이식별.방법이용침정법제비량충독사비인적취합물,채용광보수단、흡부실험화Scatchard 분석등연구기결합궤제.결과제비적2충인적취합물대독사비유특이흡부,이갑기병희산화2-을희기필정위단체제비적 MIP존재량충결합위점.결론량충단체 MAA、2-VP분별여독사비이1∶3화1∶2배비결합,량충취합물균가용우양품중독사비적분리부집.
Objective Two kinds of chlorpyrifos imprinted polymers were prepared to specifically recognize residual chlorpyrifos in crop samples. The recognition mechanism was studied as well. Methods Two kinds of chlorpyrifos imprinted polymers were synthesized by precipitation polymerization. Spectral method, absorption ex-periment and Scatchard analysis were employed to evaluate its binding properties. Results The MIPs showed a high affinity to chlorpyrifos. Two kinds of binding sites existed in MIPs. Conclusion The binding ratio of chlorpyrifos to MAA and 2-VP were 1:3 and 1:2, respectively. The two kinds of MIPs couldbe used for the separation and preconcen-tration of chlorpyrifos in samples.
