CAJ | 학술논문

目的建立动物源食品中阿布拉霉素的LC-MS/MS检测方法.方法待测样品中的阿布拉霉素用5%的三氯乙酸溶液提取,经MCX固相萃取柱净化、浓缩、定容后采用液相色谱-串联质谱法在正离子模式下检测.结果该方法在0~5 mg/L范围内有良好的线性关系,相关系数r>0.995,在不同基质中,不同的添加水平下该方法的平均回收率范围在80.9%~101%,相对标准偏差为1.17%~12.60%;定量下限为0.030 mg/kg.结论此方法灵敏度高、准确、重现性好,适用于动物组织中阿布拉霉素残留量的检测.
목적건립동물원식품중아포랍매소적LC-MS/MS검측방법.방법대측양품중적아포랍매소용5%적삼록을산용액제취,경MCX고상췌취주정화、농축、정용후채용액상색보-천련질보법재정리자모식하검측.결과해방법재0~5 mg/L범위내유량호적선성관계,상관계수r>0.995,재불동기질중,불동적첨가수평하해방법적평균회수솔범위재80.9%~101%,상대표준편차위1.17%~12.60%;정량하한위0.030 mg/kg.결론차방법령민도고、준학、중현성호,괄용우동물조직중아포랍매소잔류량적검측.
Objective To establish a method for the determination of apramycin in animal tissues by LC-MS/MS. Methods The residues of apramycin in the test samples were extracted with 5% trichloroacetic acid solution. After being cleaned up with SPE column, concentrated and reconstituted, the residues were detected by liquid chromatography-mass spectrometry under multiple reaction monitoring (MRM) mode via positive-ion mode. Results The method showed a good linearity over the range of 0~5 mg/L for apramycin with r>0.995. The average recoveries were 80.9%~101%at three or four spiked levels in animal tissues, and the relative standard devia-tions were 1.17%~12.6%. The limit of quantitation was 0.030 mg/kg. Conclusion This method is highly sensi-tive, accurate and reproducible, and it is suitable for the detection of apramycin in animal tissues.