CAJ | 학술논문

目的建立固相萃取-高压液相色谱法检测体外消化模型消化液及固相残渣中邻苯二甲酸酯(PAEs)的方法.方法食品经体外消化模型处理后离心分为固液两相,分别经溶剂提取、固相萃取柱净化后采用HPLC检测两相中的PAEs含量,通过计算得到生物有效性值.结果该方法在0.1~20 mg/L范围内线性关系良好,相关系数均大于0.998,方法的回收率在70.38%~96.49%之间,相对标准偏差均低于7%.结论该方法操作简单,灵敏度和重现性好,抗干扰能力强,可用于食品中PAEs生物有效性的测定,同时为进一步进行PAEs暴露评估和健康风险评估奠定基础.
목적건립고상췌취-고압액상색보법검측체외소화모형소화액급고상잔사중린분이갑산지(PAEs)적방법.방법식품경체외소화모형처리후리심분위고액량상,분별경용제제취、고상췌취주정화후채용HPLC검측량상중적PAEs함량,통과계산득도생물유효성치.결과해방법재0.1~20 mg/L범위내선성관계량호,상관계수균대우0.998,방법적회수솔재70.38%~96.49%지간,상대표준편차균저우7%.결론해방법조작간단,령민도화중현성호,항간우능력강,가용우식품중PAEs생물유효성적측정,동시위진일보진행PAEs폭로평고화건강풍험평고전정기출.
Objective An solid-phase extraction-high pressure liuid chromatograpy (SPE-HPLC) method was developed for determination of four kinds of phthalate acid esters(PAEs) in liquid phase and solid phase of digestive juice base on an in vitro digestion model. Methods The food sample was treated via the in vitro di-gestion model, and the digestive juices were centrifuged to separate supernatant and solid. After extracted using n-hexane and purified by SPE (Florisil column), PAEs in the liquid phase and solid phase were determined by HPLC, respectively, and then the bioaccessibility were calculated. Results The method showed a good linear-ity over the range of 0.1~20.0 mg/L for PAEs with r=0.998. The recoveries were in range of 70.38%~96.49%, and the relative standard deviations(RSDs) were lower than 7%. Conclusion The proposed method with strong anti-interference capacity is convenient, sensitive and accurate, and it is suitable for the determination of bioaccessibility of PAEs in food, and provides a way for exposure estimation and risk assessment.