CAJ | 학술논문

目的建立小麦中脱氧雪腐镰刀菌烯醇(deoxynivalenol, DON)含量测定的酶联免疫吸附(ELISA)分析方法.方法选取山东省4地区小麦样品80份,用不同溶剂对小麦中的DON进行提取后,构建小麦中DON的ELISA检测方法,根据国家标准对检测结果进行评价.结果选用84%的乙腈水溶液作为提取溶剂,其平均回收率可达100.2%,较为理想.该 ELISA 方法检测小麦中 DON,线性范围是5~5000 ng/g,回归方程 Y=0.0419X+0.0222,相关系数r=0.9868,样品中DON含量为5.27~3639.38 ng/g ,中位数为79.25 ng/g,在所检测的80份样品中,有4份超出了国家规定的上限标准(1000 ng/g),76份符合国家规定,合格率为95%.结论该方法灵敏、快捷,适用于小麦中脱氧雪腐镰刀菌烯醇的检测.
목적건립소맥중탈양설부렴도균희순(deoxynivalenol, DON)함량측정적매련면역흡부(ELISA)분석방법.방법선취산동성4지구소맥양품80빈,용불동용제대소맥중적DON진행제취후,구건소맥중DON적ELISA검측방법,근거국가표준대검측결과진행평개.결과선용84%적을정수용액작위제취용제,기평균회수솔가체100.2%,교위이상.해 ELISA 방법검측소맥중 DON,선성범위시5~5000 ng/g,회귀방정 Y=0.0419X+0.0222,상관계수r=0.9868,양품중DON함량위5.27~3639.38 ng/g ,중위수위79.25 ng/g,재소검측적80빈양품중,유4빈초출료국가규정적상한표준(1000 ng/g),76빈부합국가규정,합격솔위95%.결론해방법령민、쾌첩,괄용우소맥중탈양설부렴도균희순적검측.
Objective To explore a method of enzyme-linked immunosorbent assay (ELISA) for determi-nation of deoxynivalenol(DON)in wheat. Methods A total of 80 wheat samples collected from 4 regions of Shandong province were extracted by different solvents, detected by ELISA and evaluated according to na-tional standard. Results Optimal DON extraction was obtained when 84%acetonitrile was used in the extrac-tion agent which could guarantee the mean extraction rate of 100.2%. The method showed a good linearity over the range of 5~5000 ng/g for deoxynivalenol and the regression equation was Y= 0.0419X+0.0222 with r=0.9868. The cotent of deoxynivalenol in wheat was 5.27~3639.38 ng/g with median of 79.25 ng/g. The con-centration of 4 samples among the totals was higher than the DON tolerance limitation of 1000 ng/g, and the qualified rate was 95%. Conclusion The method is accurate and efficient and it is suitable for the determina-tion of DON in wheat.