CAJ | 학술논문

目的建立同时测定高脂肪食品中黄色2G、奶油黄、柑橘红2和苏丹红Ⅰ~Ⅳ的HPLC-DAD法.方法样品经环已烷/乙酸乙酯(1∶1, v/v)萃取, Bio-Beads SX3凝胶层析柱净化去除油脂、天然色素等大分子干扰物质.采用C18色谱柱,以甲醇和酸性水溶液(pH 4.0)为流动相梯度洗脱,二极管阵列检测器多波长同时检测这七种色素.结果在1.0~10.0 mg/L范围内,峰面积与进样量呈良好的线性关系,线性相关系数大于0.999,回收率在81.0%~98.2%,相对标准偏差在0.89%~5.21%.黄色2G、奶油黄、柑橘红2的检出限均达到1.0 mg/kg,苏丹红Ⅰ~Ⅳ色素的检出限均达到2.0 mg/kg.结论该方法简便、快捷、灵敏、准确、重现性好,能满足相关色素同时检测的需要.
목적건립동시측정고지방식품중황색2G、내유황、감귤홍2화소단홍Ⅰ~Ⅳ적HPLC-DAD법.방법양품경배이완/을산을지(1∶1, v/v)췌취, Bio-Beads SX3응효층석주정화거제유지、천연색소등대분자간우물질.채용C18색보주,이갑순화산성수용액(pH 4.0)위류동상제도세탈,이겁관진렬검측기다파장동시검측저칠충색소.결과재1.0~10.0 mg/L범위내,봉면적여진양량정량호적선성관계,선성상관계수대우0.999,회수솔재81.0%~98.2%,상대표준편차재0.89%~5.21%.황색2G、내유황、감귤홍2적검출한균체도1.0 mg/kg,소단홍Ⅰ~Ⅳ색소적검출한균체도2.0 mg/kg.결론해방법간편、쾌첩、령민、준학、중현성호,능만족상관색소동시검측적수요.
Objective To establish an HPLC-DAD method for simultaneous determination of yellow 2G, butter yellow, citrus red 2 and sudan red I~IV. Methods Samples were extracted directly with a mixture of cyclohexane-athylacetate (1∶1, v/v ) and purified by a Bio-beads SX3 gel column, to remove interfering high molecular fatty substances and natural pigments in food samples. HPLC was carried out on a C18 column with MeOH+H2O (pH 4.0) as mobile phase in the gradient elution. Multiwavelength diode array detection (DAD) was applied in the determination of the 7 kinds of pigments. Results Good linear relationships in the range of 1.0~10 mg/L of 7 compounds were obtained with correlation coefficients (r) all above 0.999. The RSDs were in the range of 0.89%~5.21%, and average recoveries were 81.0%~98.2%. The detection limits were 1.0 mg/kg for yellow 2G, butter yellow, citrus red 2 and 2.0 mg/kg for sudan red I~IV, respectively. Conclusion The established method is simple, rapid, sensitive, accurate and repeatable, and can be used for the simultaneous determination of relevant pigments.