CAJ | 학술논문

目的探讨他克莫司后处理能否诱导大鼠缺血脊髓对再灌注损伤的耐受.方法成年雄性SD大鼠30只,随机分为假手术(SO)组、缺血再灌注(IR)组和他克莫司后处理(TP)组,每组10只大鼠,采用经股动脉置管球囊扩张制备脊髓缺血模型,SO 组仅行置管,IR 组在脊髓缺血20分钟后行再灌注,TP 组在脊髓缺血20分钟后再灌注,即刻经左颈总动脉一次性注射他克莫司0.5mg/kg.再灌注后7、14天采用 Tarlov 评分法检测大鼠后肢运动功能,脊髓组织切片HE染色观察病理学改变.结果SO组大鼠各时间点后肢Tarlov评分均为5分,形态学检测显示脊髓组织结构正常;IR组大鼠Tarlov评分明显降低,脊髓组织呈现出坏死、水肿、空腔形成等缺血再灌注损伤表现;TP组大鼠Tarlov评分结果显著优于IR组,脊髓组织病理变化较IR组为轻.结论建立大鼠脊髓缺血再灌注损伤模型,并初步证实他克莫司后处理能诱导缺血脊髓对再灌注损伤的耐受.
목적탐토타극막사후처리능부유도대서결혈척수대재관주손상적내수.방법성년웅성SD대서30지,수궤분위가수술(SO)조、결혈재관주(IR)조화타극막사후처리(TP)조,매조10지대서,채용경고동맥치관구낭확장제비척수결혈모형,SO 조부행치관,IR 조재척수결혈20분종후행재관주,TP 조재척수결혈20분종후재관주,즉각경좌경총동맥일차성주사타극막사0.5mg/kg.재관주후7、14천채용 Tarlov 평분법검측대서후지운동공능,척수조직절편HE염색관찰병이학개변.결과SO조대서각시간점후지Tarlov평분균위5분,형태학검측현시척수조직결구정상;IR조대서Tarlov평분명현강저,척수조직정현출배사、수종、공강형성등결혈재관주손상표현;TP조대서Tarlov평분결과현저우우IR조,척수조직병리변화교IR조위경.결론건립대서척수결혈재관주손상모형,병초보증실타극막사후처리능유도결혈척수대재관주손상적내수.
Objective To investigate whether tacrolimus postconditioning could induce tolerance of ischemic spinal cord to reperfusion injury in rats. Methods Thirty male SD rats were randomly divided into sham operation (SO) group, ischemia-reperfusion (IR) group and tacrolimus postconditioning (TP) group with 10 rats in each group. The model of spinal cord ischemia was prepared by means of catheterization through femoral artery and balloon dilatation. SO group received catheterization only. IR group underwent reperfusion 20 minutes after spinal cord ischemia. TP group experi-enced a single injection of tacrolimus (0.5mg/kg) through the left common carotid artery at the onset of reperfusion. At 7 and 14 days after reperfusion, hindlimb motor function was detected by using Tarlov scale and pathological changes in spinal cord tissue sections were observed through HE staining. Results In SO group, Tarlov scores were 5 and the spinal cord tissues remained intact at each time point. Tarlov scores of IR group dropped remarkably and the spinal cord tissues demonstrated lesions such as necrosis, edema and cavity formation. Tarlov scores were significantly higher in TP group than in IR group, and pathological changes in TP group were not as serious as those in IR group. Conclusion We suc-cessfully establish the model of spinal cord ischemia-reperfusion injury in rats and initially confirm that tacrolimus post-conditioning can induce tolerance of ischemic spinal cord to reperfusion injury.