CAJ | 학술논문

目的:探讨促吞噬肽衍生物 T 肽抑制裸鼠术后残瘤生长的作用机理.方法:建立 MCF-7乳腺癌裸鼠皮下移植瘤术后残瘤模型,观察8 mg/kg 剂量 T 肽对残余肿瘤组织的生长情况,并采用免疫组化和 Western 印迹检测给药后残瘤组织血管内皮生长因子(VEGF)的表达,采用 RT-PCR 检测不同 T 肽浓度对血管内皮细胞 VEGF 基因转录的影响.结果:T 肽对 MCF-7乳腺癌裸鼠皮下移植瘤术后残瘤生长表现出良好的抑制作用,按照瘤重得出的抑制率为68.2%,按照肿瘤体积得出的抑制率为67.6%,T 肽给药组裸鼠残瘤组织中 VEGF 的表达量较空白对照组明显减少.血管内皮细胞中 T 肽呈剂量相关性抑制 VEGF 基因的转录.结论:T 肽的抗癌作用与其抑制肿瘤微环境肿瘤组织和血管内皮细胞中 VEGF 的表达有密切联系,预示着 T 肽有潜力成为预防术后残瘤生长的抗癌新药.
목적:탐토촉탄서태연생물 T 태억제라서술후잔류생장적작용궤리.방법:건립 MCF-7유선암라서피하이식류술후잔류모형,관찰8 mg/kg 제량 T 태대잔여종류조직적생장정황,병채용면역조화화 Western 인적검측급약후잔류조직혈관내피생장인자(VEGF)적표체,채용 RT-PCR 검측불동 T 태농도대혈관내피세포 VEGF 기인전록적영향.결과:T 태대 MCF-7유선암라서피하이식류술후잔류생장표현출량호적억제작용,안조류중득출적억제솔위68.2%,안조종류체적득출적억제솔위67.6%,T 태급약조라서잔류조직중 VEGF 적표체량교공백대조조명현감소.혈관내피세포중 T 태정제량상관성억제 VEGF 기인적전록.결론:T 태적항암작용여기억제종류미배경종류조직화혈관내피세포중 VEGF 적표체유밀절련계,예시착 T 태유잠력성위예방술후잔류생장적항암신약.
Objective: To study the possible inhibition mechanisms of T peptide derived from tuftsin on growth of residual tumors in nude mice after surgery. Methods: The nude mice were implanted breast tumor cell line MCF-7 to allow the tumor to grow, and then they were operated to prepare the mice models with residual tumor. The inhibitory effect of T peptide on post-operative tumors was studied by measuring the tumor tissues size of the mice model receiving 8 mg/kg T peptide every 2 days for 10 days. And expression of vascular endothelial growth factor(VEGF) was detected in these tumor tissues by Western blot and immunohistochemistry. The VEGF gene transcriptions in human umbilical vein endothelial cells(HUVEC) treated by different concentration T peptide were detected by RT-PCR. Results: In post-surgical residual tumors, T peptide produced strong inhibitory effect with tumor weight suppression rate of 68.2% and tumor volume inhibition rate of 67.6% . The protein expression of VEGF in residual tumor tissues of T peptide group was significantly lower than that of control group. The mRNA level of VEGF in HUVEC in vitro decreased by T peptide with a dose-dependent manner. Conclusion: Inhibiting the expression of VEGF in tumor is the mechanism of antitumor effects on the post-surgical residual tumors in mice by T peptide. So T peptide might be a promising candidate drug for the remedy for post-surgical residual tu?mors.