CAJ | 학술논문

目的:比较猴 B 病毒血清抗体和病毒 PCR 检测结果,阐明动物感染后病毒在机体内的存在状况.方法:采集成年猴血清和三叉神经组织,首先通过 ELISA 方法检测血清中 B 病毒抗体,然后采用 B 病毒 gL 和 gD 基因引物通过PCR 方法扩增血清 DNA 和三叉神经组织 DNA,比较2种方法的检测结果,并对扩增产物进行序列分析.结果:22份猴血清中,B 病毒抗体呈阳性的有13份(59.1%);PCR 结果显示,抗体阴性动物及所有血清 DNA 模板中均无阳性扩增,但在13份抗体阳性动物的三叉神经组织 DNA 样品中,PCR 阳性4份(30.8%);gL 和 gD 基因扩增条件及产物分析表明,gL 基因的 GC 含量为64.1%,gD 为74.2%,且 gL 的扩增条件和效果明显优于 gD.结论:B 病毒感染猴后,将在部分动物神经节中建立潜伏,而 gL 基因更适合作为分子鉴定的靶标.
목적:비교후 B 병독혈청항체화병독 PCR 검측결과,천명동물감염후병독재궤체내적존재상황.방법:채집성년후혈청화삼차신경조직,수선통과 ELISA 방법검측혈청중 B 병독항체,연후채용 B 병독 gL 화 gD 기인인물통과PCR 방법확증혈청 DNA 화삼차신경조직 DNA,비교2충방법적검측결과,병대확증산물진행서렬분석.결과:22빈후혈청중,B 병독항체정양성적유13빈(59.1%);PCR 결과현시,항체음성동물급소유혈청 DNA 모판중균무양성확증,단재13빈항체양성동물적삼차신경조직 DNA 양품중,PCR 양성4빈(30.8%);gL 화 gD 기인확증조건급산물분석표명,gL 기인적 GC 함량위64.1%,gD 위74.2%,차 gL 적확증조건화효과명현우우 gD.결론:B 병독감염후후,장재부분동물신경절중건립잠복,이 gL 기인경괄합작위분자감정적파표.
Objective: To elucidate the state of monkey B virus(BV) in infected monkey, antibodies and viral DNA in 22 monkeys were detected at the same time. Methods: Sera and trigeminal ganglia(TG) tissue were col?lected from 22 adult monkeys. At first, antibodies to BV in sera were detected by dot immunobinding assay (DIA). Then, DNA was extracted and purified from sera and TG tissue and used as templates for PCR with prim?ers on gL or gD gene of BV. Products of PCR were sequenced and blast in GenBank. Results: In 22 monkey se?ra, 13(59.1%) were confirmed for the presence of antibodies to BV. Results of PCR showed that the virus DNA was present in TG of 4 of the 13 seropositve monkeys(30.8%) tested, but no virus being detected in all sera sam?ples. Sequencing products of PCR showed G+C content in gD gene was very high(74.2%) and middle in gL gene (64.1%). The target of gL gene for PCR was superior to gD gene. Conclusion: Our results indicated that B vi?rus would establish latent in trigeminal ganglia only in part of infected moneys and gL gene locus of BV is a suit?able target for specific and rapid identification of viral infection by PCR technology.