CAJ | 학술논문

目的:通过比较添加不同浓度的无血清培养添加剂 N2、B27对神经干细胞增殖的影响,探讨一种最优的体外培养大鼠胚胎神经干细胞的方法.方法:分离培养胚胎13.5d SD 大鼠的神经干细胞,采用 CCK-8细胞计数、BrdU 掺入、测量神经球直径的方法,比较不同浓度 N2、B27对神经干细胞增殖的影响.结果:N2与 B27合用对神经干细胞增殖和成球的作用最为明显,单独添加2% B27的作用优于1% B27和 N2组.结论:1% N2和1% B27同时添加更有利于神经干细胞的增殖和自我更新,此方法为神经干细胞的应用提供了实验依据.
목적:통과비교첨가불동농도적무혈청배양첨가제 N2、B27대신경간세포증식적영향,탐토일충최우적체외배양대서배태신경간세포적방법.방법:분리배양배태13.5d SD 대서적신경간세포,채용 CCK-8세포계수、BrdU 참입、측량신경구직경적방법,비교불동농도 N2、B27대신경간세포증식적영향.결과:N2여 B27합용대신경간세포증식화성구적작용최위명현,단독첨가2% B27적작용우우1% B27화 N2조.결론:1% N2화1% B27동시첨가경유리우신경간세포적증식화자아경신,차방법위신경간세포적응용제공료실험의거.
Objective: In order to find an optimal method to cultivate embryonic neural stem cells in vitro, we compared the effect of different supplement N2 and B27 with different concentrations on the proliferation of neural stem cells. Methods: The embryonic neural stem cells were isolated from 13.5 d SD rat. The effects of different supplements N2 and B27 with different concentrations on the proliferation of neural stem cells were investigated and compared by the methods of CCK-8 counting, BrdU administration and measuring neural ball diameter. Re?sults: The combination group of N2 and B27 had most obvious effect on the proliferation and formation of neuro?spheres of neural stem cells; the effect of 2% B27 alone was better than that of 1% B27 group and N2 group. Conclusion: The combined supplement of 1% N2 and 1% B27 were more conductive to the proliferation and self-renewal of neural stem cells, it will provide experimental bases for the application of neural stem cells.