CAJ | 학술논문

目的:针对分子育种工作的繁琐性及葱属植物次生代谢物较多的特点,研究一种利用普通试剂及简单仪器高效快速提取葱属植物 DNA 的方法.方法:对碱处理法稍加改进,在碱性环境中高温裂解细胞,将释放的 DNA 保存在 Tris 缓冲液中,用 PCR 检测提取质量并分析 DNA 的保存时间.结果:与用试剂盒提取的 DNA 相比,扩增产物无显著差异,利用此方法提取 DNA 并分析了13个洋葱品种的细胞质雄性不育类型;保存时间分析显示,DNA 在常温下只能保存5 d,在4℃或-20℃可至少保存2个月.结论:该方法方便快速、成本低、适于田间操作,得到的 DNA 可满足分子生物学实验的基本要求,可用于以 PCR 为基础的分子标记辅助育种.
목적:침대분자육충공작적번쇄성급총속식물차생대사물교다적특점,연구일충이용보통시제급간단의기고효쾌속제취총속식물 DNA 적방법.방법:대감처리법초가개진,재감성배경중고온렬해세포,장석방적 DNA 보존재 Tris 완충액중,용 PCR 검측제취질량병분석 DNA 적보존시간.결과:여용시제합제취적 DNA 상비,확증산물무현저차이,이용차방법제취 DNA 병분석료13개양총품충적세포질웅성불육류형;보존시간분석현시,DNA 재상온하지능보존5 d,재4℃혹-20℃가지소보존2개월.결론:해방법방편쾌속、성본저、괄우전간조작,득도적 DNA 가만족분자생물학실험적기본요구,가용우이 PCR 위기출적분자표기보조육충.
Objective: Because breeding is tedious and Allium have many secondary metabolites, we described a rapid method using common reagents and simple apparatus for extracting DNA in Allium. Methods: The alkali treatment was improved. The plant cells were cracked in the high temperature when exist NaOH, then the re?leased DNA were stored in Tris buffer. The quality and storage period of DNA were studied by PCR. Results:Compared with DNA extracted by kit, their amplification product have no significant difference. Using this method the DNA were extracted and the styles of cytoplasm were identified of 13 onion varieties. Analysis of the time of storage showed that DNA can be kept for 5 days at room temperature, at least two months at 4℃ or -20℃. Con?clusion: This rapid method is convenient and fast. Also, it has the low cost and is suitable for field operation. The extracted DNA can be used for the molecular marker-assisted breeding based on PCR.