CAJ | 학술논문

近年来,用于重组蛋白生产的哺乳动物细胞表达领域涌现出一系列革命性的新技术.优化的工程细胞为表达重组蛋白提供了优良的宿主;基于荧光的筛选方法可以快捷地得到高表达细胞株;高通量的培养工艺能够预测适合外源蛋白表达的细胞培养条件;可抛弃式生物反应器为大规模细胞培养提供了更多的选择;大规模瞬时表达技术节省了重组蛋白的生产时间.这些新技术提高了重组蛋白的研发和生产效率,加快了蛋白药物的工业化进程.
근년래,용우중조단백생산적포유동물세포표체영역용현출일계렬혁명성적신기술.우화적공정세포위표체중조단백제공료우량적숙주;기우형광적사선방법가이쾌첩지득도고표체세포주;고통량적배양공예능구예측괄합외원단백표체적세포배양조건;가포기식생물반응기위대규모세포배양제공료경다적선택;대규모순시표체기술절성료중조단백적생산시간.저사신기술제고료중조단백적연발화생산효솔,가쾌료단백약물적공업화진정.
In recent years, some revolutionary new technologies in mammalian cell expression field have been developed for industrial recombinant protein production. Optimized engineered cells were applied to express recom?binant protein as more suitable hosts. High-expressing cell lines can be easily obtained through fluores?cence-based screening methods. The best conditions for expression of heterologous proteins were predicted by high-throughput culture platform. Disposable bioreactors can be developed as alternative facilities for large-scale cell culture. The manufacture period of recombinant proteins was saved due to large-scale transient expression technology. By means of these technologies, the efficiency of research and production was greatly improved to ac?celerate industrialization of protein drug.