CAJ | 학술논문

目的:从腾冲热海温泉中分离嗜热芽孢杆菌噬菌体,并初步分析其特征.方法:采用双层平板法分离纯化嗜热芽孢杆菌噬菌体,对分离得到的噬菌体进行电镜形态观察,按照感染复数(MOI)分别为0.01、0.1、1.0、10和100加入噬菌体纯培养液和宿主菌,55℃、160 r/min培养8 h后测定噬菌体滴度,并进行噬菌体的热稳定性和pH稳定性分析.结果:从腾冲热海温泉中分离得到的噬菌体为二十面体型;其感染宿主菌NHH4形成清晰的噬菌斑,最适MOI为1.0,最适感染温度为55℃,最适感染pH值为7.5.将这株噬菌体命名为TBIP1.结论:从腾冲热海温泉中分离得到的噬菌体TBIP1为典型的二十面体型,当MOI为1.0时,TBIP1感染其宿主菌产生的子代噬菌体滴度最高.
목적:종등충열해온천중분리기열아포간균서균체,병초보분석기특정.방법:채용쌍층평판법분리순화기열아포간균서균체,대분리득도적서균체진행전경형태관찰,안조감염복수(MOI)분별위0.01、0.1、1.0、10화100가입서균체순배양액화숙주균,55℃、160 r/min배양8 h후측정서균체적도,병진행서균체적열은정성화pH은정성분석.결과:종등충열해온천중분리득도적서균체위이십면체형;기감염숙주균NHH4형성청석적서균반,최괄MOI위1.0,최괄감염온도위55℃,최괄감염pH치위7.5.장저주서균체명명위TBIP1.결론:종등충열해온천중분리득도적서균체TBIP1위전형적이십면체형,당MOI위1.0시,TBIP1감염기숙주균산생적자대서균체적도최고.
Objective: To isolate the thermophilic Bacillus phages from Tengchong hot springs and to analyze its characteristics. Methods: Double-layer plate was adopted to isolate thermophilic Bacillus phages from the hot springs and one strain was selected to analyze its morphologic characteristics, then culture liquid and host bacteri?um of Bacillus were added according to MOI at 0.01, 0.1, 1.0, 10 and 100. Titer was tested and the stabilities of Bacillus and pH were analyzed after the developing at 55℃, 160 r/min for 8 hours. Results: The thermophilic Ba?cillus phage TBIP1 was an icosahedral phage, after infecting its host, clear plaques could be observed. The MOI of TBIP1 was 1.0. The infection temperature and pH value of TBIP1 were 45~65℃ and 5.0~10.0, respectively, with optimal temperature and pH at 55℃ and 7.5. Conclusion: TBIP1 is typical icosahedral phage. When MOI is at 1.0, the titer of its progeny Bacillus which is produced from its affected host is the highest.