CAJ | 학술논문

目的:在SDS-PAGE方法的基础上建立一种改进方法,用于流感病毒亚单位疫苗中间体的杂质分析.方法:用糖苷酶F对不同亚型的疫苗中间体去糖基化处理后进行SDS-PAGE,与未经去糖基化处理的还原型SDS-PAGE结果进行比较.结果:去糖基化处理消除了血凝素的糖基化对电泳迁移率的影响,从而实现了血凝素亚基和杂质的电泳分离.结论:此方法应用于流感病毒亚单位疫苗中间体的杂质分析,较之常规的还原型SDS-PAGE方法具有更高的分辨力.
목적:재SDS-PAGE방법적기출상건립일충개진방법,용우류감병독아단위역묘중간체적잡질분석.방법:용당감매F대불동아형적역묘중간체거당기화처리후진행SDS-PAGE,여미경거당기화처리적환원형SDS-PAGE결과진행비교.결과:거당기화처리소제료혈응소적당기화대전영천이솔적영향,종이실현료혈응소아기화잡질적전영분리.결론:차방법응용우류감병독아단위역묘중간체적잡질분석,교지상규적환원형SDS-PAGE방법구유경고적분변력.
Objective: To establish an improved method of impurity analysis based on SDS-PAGE for the inter?mediate of influenza subunit vaccine. Methods: Different types of vaccine intermediates were deglycosylated using PNGase F followed by SDS-PAGE. Deglycosylated and non-deglycosylated samples were analyzed by SDS-PAGE. Results: Deglycosylating eliminate the effects of electrophoretic mobility by haemagglutinin glycosylation. Therefore, subunits of haemagglutinin could be separated from impurities satisfactorily by electrophoresis. Conclusion: This improved method provided a higher resolution in the impurity analysis for influenza subunit vaccine intermediate, in comparison of regular reduced SDS-PAGE.