CAJ | 학술논문

近年来毕赤酵母已成为一种优越的异源蛋白表达系统.而提高目的蛋白的表达水平,高密度发酵已成为关键技术环节之一.我们从毕赤酵母工程菌的选择、培养基的优化设计,以及发酵工程过程控制等方面简要阐述毕赤酵母的高密度发酵,并提出了工程菌在高密度发酵过程中存在的问题.
근년래필적효모이성위일충우월적이원단백표체계통.이제고목적단백적표체수평,고밀도발효이성위관건기술배절지일.아문종필적효모공정균적선택、배양기적우화설계,이급발효공정과정공제등방면간요천술필적효모적고밀도발효,병제출료공정균재고밀도발효과정중존재적문제.
Pichia pastoris has been utilized widely as an excellent heterologous gene expression system recently. High-density fermentation has been a key technique tache to improve the expression level of the protein of inter?est. In this paper, we expounded the choose of engineering bacteria, designation and optimization of culture medi?um, and control of fermentation course to increase P.pastoris on high-density fermentation. Moreover, the questions existing in industry high-density fermentation were put forward.