CAJ | 학술논문

目的观察不同年龄段慢性肝衰竭患者自体骨髓间充质干细胞体外增殖能力的特点.方法在慢性肝衰竭患者30例和非肝病患者30例,各分3个组,即A组为20~35岁,B组为35~55岁和C组为>55岁.取骨髓血50毫升,采用密度梯度离心法分离出单个核细胞,接种于56.7cm2培养皿内,观察细胞生长情况,并在第7天计数集落形成单位;当贴壁细胞融合后进行传代,计算增至治疗所需细胞数量的传代时间和细胞增殖率.结果在慢性肝衰竭患者,A组为6例,B组为18例,C组为6例;在非肝病组,分别为7、12和11例;在肝衰竭患者A组、B组和C组每皿细胞集落单位分别为15.3±8.7×106、8.6±5.2×106和4.2±3.6×106,而在非肝病患者则分别为14.7±7.1×106、13.5±7.4×106和10.3±4.9×106(P<0.05);第一代细胞倍增时间比较,肝衰竭患者A组、B组和C组分别为178.7±79.6h、264.1±102.3h和457.7±155.6h,而非肝病患者则分别为156.3±62.5h、192.7±88.6h和311.6±142.8h(P<0.05);第二代细胞倍增时间比较,肝衰竭患者也明显比非肝病患者延长.结论大于55岁的慢性肝衰竭患者自体骨髓干细胞体外增殖能力明显降低.在选择干细胞移植治疗时,宜选择其他来源的细胞.
목적관찰불동년령단만성간쇠갈환자자체골수간충질간세포체외증식능력적특점.방법재만성간쇠갈환자30례화비간병환자30례,각분3개조,즉A조위20~35세,B조위35~55세화C조위>55세.취골수혈50호승,채용밀도제도리심법분리출단개핵세포,접충우56.7cm2배양명내,관찰세포생장정황,병재제7천계수집락형성단위;당첩벽세포융합후진행전대,계산증지치료소수세포수량적전대시간화세포증식솔.결과재만성간쇠갈환자,A조위6례,B조위18례,C조위6례;재비간병조,분별위7、12화11례;재간쇠갈환자A조、B조화C조매명세포집락단위분별위15.3±8.7×106、8.6±5.2×106화4.2±3.6×106,이재비간병환자칙분별위14.7±7.1×106、13.5±7.4×106화10.3±4.9×106(P<0.05);제일대세포배증시간비교,간쇠갈환자A조、B조화C조분별위178.7±79.6h、264.1±102.3h화457.7±155.6h,이비간병환자칙분별위156.3±62.5h、192.7±88.6h화311.6±142.8h(P<0.05);제이대세포배증시간비교,간쇠갈환자야명현비비간병환자연장.결론대우55세적만성간쇠갈환자자체골수간세포체외증식능력명현강저.재선택간세포이식치료시,의선택기타래원적세포.
Objective To observe the proliferative capacity of human bone marrow mesenchymal stem cells (MSCs) in patients with chronic liver failure in vitro. Methods MSCs were isolated from human bone marrow from 30 patients with chronic liver failure and 30 patients with benign diseases. In each group,the patients were divided into group A(younger than 30 year old),group B(aged from 31 to 55 year old)and group C(older than 55 years). The cell numbers were determined at the end of every passage,and the capability of proliferation was calculated by colony-forming units and double times at the first and second passage. Results There were six in group A, eighteen in group B and six in group C in patients with chronic liver failure,and they were seven, twelve and eleven patients with benign diseases;the colony-forming units in group A,B and C in patients with liver failure were 15.3±8.7×106,8.6±5.2×106 and 4.2±3.6×106,respectively,while in patients with benign diseases, they were 14.7±7.1×106,13.5±7.4×106 and 10.3±4.9×106,respectively (P<0.05);the double times of first passage in group A,B and C in patients with liver failure were 178.7±79.6h,264.1±102.3h and 457.7±155.6h,respectively, while they were 156.3±62.5h,192.7±88.6h and 311.6±142.8h, respectively (P<0.05);in second passage,the double times in patients with liver failure prolonged obviously too, as compared to those in patients with benign diseases. Conclusion The proliferation capability of MSCs decreases in patients with chronic liver failure,especially in el-derly patients.